PKM2 regulates the Warburg effect and promotes HMGB1 release in sepsis

Liangchun Yang, Min Xie, Minghua Yang, Yan Yu, Shan Zhu, Wen Hou, Rui Kang, Michael T. Lotze, Timothy R. Billiar, Haichao Wang, Lizhi Cao, Daolin Tang

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

Increasing evidence suggests the important role of metabolic reprogramming in the regulation of the innate inflammatory response, but the underlying mechanism remains unclear. Here we provide evidence to support a novel role for the pyruvate kinase M2 (PKM2)-mediated Warburg effect, namely aerobic glycolysis, in the regulation of high-mobility group box 1 (HMGB1) release. PKM2 interacts with hypoxia-inducible factor 1α (HIF1α) and activates the HIF-1α -dependent transcription of enzymes necessary for aerobic glycolysis in macrophages. Knockdown of PKM2, HIF1α and glycolysis-related genes uniformly decreases lactate production and HMGB1 release. Similarly, a potential PKM2 inhibitor, shikonin, reduces serum lactate and HMGB1 levels, and protects mice from lethal endotoxemia and sepsis. Collectively, these findings shed light on a novel mechanism for metabolic control of inflammation by regulating HMGB1 release and highlight the importance of targeting aerobic glycolysis in the treatment of sepsis and other inflammatory diseases.

Original languageEnglish (US)
Article number4436
JournalNature communications
Volume5
DOIs
StatePublished - Jul 14 2014
Externally publishedYes

Fingerprint

glycolysis
pyruvates
Pyruvate Kinase
Glycolysis
boxes
Sepsis
Hypoxia-Inducible Factor 1
lactates
hypoxia
Lactic Acid
Endotoxemia
macrophages
Macrophages
Transcription
genes
serums
inhibitors
mice
enzymes
Genes

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

PKM2 regulates the Warburg effect and promotes HMGB1 release in sepsis. / Yang, Liangchun; Xie, Min; Yang, Minghua; Yu, Yan; Zhu, Shan; Hou, Wen; Kang, Rui; Lotze, Michael T.; Billiar, Timothy R.; Wang, Haichao; Cao, Lizhi; Tang, Daolin.

In: Nature communications, Vol. 5, 4436, 14.07.2014.

Research output: Contribution to journalArticle

Yang, L, Xie, M, Yang, M, Yu, Y, Zhu, S, Hou, W, Kang, R, Lotze, MT, Billiar, TR, Wang, H, Cao, L & Tang, D 2014, 'PKM2 regulates the Warburg effect and promotes HMGB1 release in sepsis', Nature communications, vol. 5, 4436. https://doi.org/10.1038/ncomms5436
Yang, Liangchun ; Xie, Min ; Yang, Minghua ; Yu, Yan ; Zhu, Shan ; Hou, Wen ; Kang, Rui ; Lotze, Michael T. ; Billiar, Timothy R. ; Wang, Haichao ; Cao, Lizhi ; Tang, Daolin. / PKM2 regulates the Warburg effect and promotes HMGB1 release in sepsis. In: Nature communications. 2014 ; Vol. 5.
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