Placental thromboxane and prostacyclin production in an ovine diabetic model

Jan E. Dickinson, Bruce A. Meyer, Peter C. Brath, Susie Chmielowiec, Scott W. Walsh, Valerie M. Parisi, Sue M. Palmer

Research output: Contribution to journalArticle

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Abstract

We hypothesized that streptozocin-induced ovine diabetes would cause alterations in the placental production of thromboxane and prostacyclin. With a tissue incubation technique, we examined the placental production of thromboxane and prostacyclin in cotyledons from seven normal near-term ewes (127 ± 3 days' gestation) and six streptozocin-induced diabetic ewes (125 ± 3 days' gestation). Diabetic status was verified with serial fasting blood glucose assessments. Placental tissue was incubated in Dulbecco's modified Eagle's medium for 48 hours at 37° C with 95% oxygen and 5% carbon dioxide. Samples were collected at 0, 1, 2, 4, 8, 20, 32, and 48 hours. Radioimmunoassay of the stable metabolites thromboxane B2 and 6-keto-prostaglandin F were used to determine thromboxane and prostacyclin production, respectively. Placental thromboxane production was reduced in diabetic animals when compared with control animals (5.63 ± 2.81 vs 7.32 ± 1.37 pg/mg per hour, respectively; p < 0.05). Prostacyclin production was also significantly reduced in the diabetic placentas compared with control placentas (11.44 ± 4.06 vs 16.29 ± 4.59 pg/mg per hour, respectively; p < 0.05). We conclude that the ovine placenta produces thromboxane and prostacyclin. The ovine thromboxane production rate is comparable to that of the human placenta but the prostacyclin production rate is approximately two to three times higher. The observed decrease in the placental production of thromboxane and prostacyclin may reflect an adverse effect of hyperglycemia directly on eicosanoid production or indirectly through decreased placental cellular proliferation.

Original languageEnglish (US)
Pages (from-to)1831-1835
Number of pages5
JournalAmerican Journal of Obstetrics and Gynecology
Volume163
Issue number6 PART 1
DOIs
StatePublished - 1990

Fingerprint

Thromboxanes
Epoprostenol
Sheep
Placenta
Streptozocin
Pregnancy
Thromboxane B2
Eagles
Eicosanoids
Cotyledon
Carbon Dioxide
Hyperglycemia
Radioimmunoassay
Blood Glucose
Fasting
Cell Proliferation
Oxygen

Keywords

  • diabetes
  • ovine
  • placenta
  • Prostacyclin
  • thromboxane

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Dickinson, J. E., Meyer, B. A., Brath, P. C., Chmielowiec, S., Walsh, S. W., Parisi, V. M., & Palmer, S. M. (1990). Placental thromboxane and prostacyclin production in an ovine diabetic model. American Journal of Obstetrics and Gynecology, 163(6 PART 1), 1831-1835. https://doi.org/10.1016/0002-9378(90)90759-Z

Placental thromboxane and prostacyclin production in an ovine diabetic model. / Dickinson, Jan E.; Meyer, Bruce A.; Brath, Peter C.; Chmielowiec, Susie; Walsh, Scott W.; Parisi, Valerie M.; Palmer, Sue M.

In: American Journal of Obstetrics and Gynecology, Vol. 163, No. 6 PART 1, 1990, p. 1831-1835.

Research output: Contribution to journalArticle

Dickinson, JE, Meyer, BA, Brath, PC, Chmielowiec, S, Walsh, SW, Parisi, VM & Palmer, SM 1990, 'Placental thromboxane and prostacyclin production in an ovine diabetic model', American Journal of Obstetrics and Gynecology, vol. 163, no. 6 PART 1, pp. 1831-1835. https://doi.org/10.1016/0002-9378(90)90759-Z
Dickinson, Jan E. ; Meyer, Bruce A. ; Brath, Peter C. ; Chmielowiec, Susie ; Walsh, Scott W. ; Parisi, Valerie M. ; Palmer, Sue M. / Placental thromboxane and prostacyclin production in an ovine diabetic model. In: American Journal of Obstetrics and Gynecology. 1990 ; Vol. 163, No. 6 PART 1. pp. 1831-1835.
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abstract = "We hypothesized that streptozocin-induced ovine diabetes would cause alterations in the placental production of thromboxane and prostacyclin. With a tissue incubation technique, we examined the placental production of thromboxane and prostacyclin in cotyledons from seven normal near-term ewes (127 ± 3 days' gestation) and six streptozocin-induced diabetic ewes (125 ± 3 days' gestation). Diabetic status was verified with serial fasting blood glucose assessments. Placental tissue was incubated in Dulbecco's modified Eagle's medium for 48 hours at 37° C with 95{\%} oxygen and 5{\%} carbon dioxide. Samples were collected at 0, 1, 2, 4, 8, 20, 32, and 48 hours. Radioimmunoassay of the stable metabolites thromboxane B2 and 6-keto-prostaglandin F1α were used to determine thromboxane and prostacyclin production, respectively. Placental thromboxane production was reduced in diabetic animals when compared with control animals (5.63 ± 2.81 vs 7.32 ± 1.37 pg/mg per hour, respectively; p < 0.05). Prostacyclin production was also significantly reduced in the diabetic placentas compared with control placentas (11.44 ± 4.06 vs 16.29 ± 4.59 pg/mg per hour, respectively; p < 0.05). We conclude that the ovine placenta produces thromboxane and prostacyclin. The ovine thromboxane production rate is comparable to that of the human placenta but the prostacyclin production rate is approximately two to three times higher. The observed decrease in the placental production of thromboxane and prostacyclin may reflect an adverse effect of hyperglycemia directly on eicosanoid production or indirectly through decreased placental cellular proliferation.",
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