Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain

David J. Kwiatkowski, Thomas P. Stossel, Stuart H. Orkin, John E. Mole, Harvey R. Coltens, Helen L. Yin

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Abstract

Gelsolin is representative of a class of actin-modulating proteins found in lower eukaryotes to mammals, which sever actin filaments1. Gelsolin found in the cytoplasm of cells is functionally similar to a mammalian plasma protein of similar size, originally called ADF or brevin. Human plasma and rabbit macrophage gelsolins differ by the presence of a 25-amino-acid residue extension on plasma gelsolin which appears to account for the difference in relative molecular mass (Mr) between the proteins as assessed by SDS-polyacrylamide gel electrophoresis (PAGE), 93,000 (93K) and 90K, respectively2. Here we report the isolation of full-length human plasma gelsolin complementary DNA clones from a HepG2 library. The inferred amino-acid sequence reveals the presence of a signal peptide, a long tandem repeat that matches the actin-binding domains of gelsolin, a tetrapeptide present in actin and extended regions of identical sequence with rabbit macrophage gelsolin. Southern blot analysis indicates that a single gene in the haploid genome encodes both protein forms.

Original languageEnglish (US)
Pages (from-to)455-458
Number of pages4
JournalNature
Volume323
Issue number6087
DOIs
StatePublished - Dec 1 1986

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Kwiatkowski, D. J., Stossel, T. P., Orkin, S. H., Mole, J. E., Coltens, H. R., & Yin, H. L. (1986). Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain. Nature, 323(6087), 455-458. https://doi.org/10.1038/323455a0