Plasma biomarkers in pediatric patients undergoing cardiopulmonary bypass

Melinda E. Lull, Nurgul Carkaci-Salli, Willard M. Freeman, John L. Myers, Frank M. Midgley, Neal J. Thomas, Stephen J. Kimatian, Kent E. Vrana, Akif Ündar

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

It is critical to identify at-risk patients and minimize the deleterious effects of cardiopulmonary bypass (CPB) procedures in pediatric populations. The present study screened the plasma proteome of pediatric patients undergoing CPB procedures to identify potential clinical biomarkers related to tissue damage, inflammation, or other pathologies. Blood samples were collected at five different time points from 10 children undergoing a CPB procedure. Plasma was isolated and analyzed using two-dimensional differential in-gel electrophoresis and matrix-assisted laser desorption ionization time of flight mass spectrometry. Levels of differentially regulated proteins identified by two-dimensional differential in-gel electrophoresis, and related proteins were then measured in all time points and patients. As well, associated small molecules and ions were measured. The present study identified 13 proteins and protein isoforms altered in expression, including hemopexin, ceruloplasmin, inter-alpha inhibitor H4, and alpha-2-macroglobulin. Immunoblot analysis revealed significant decreases in each of these proteins during the CPB procedure. Significant changes in the levels of copper, iron, Hb, epinephrine, norepinephrine, and serotonin were observed. The potential markers of pathology (inflammation, oxidative stress) identified during this preliminary study may illuminate opportunities for preventative measures and/or treatments during and following CPB procedures in pediatric patients.

Original languageEnglish (US)
Pages (from-to)638-644
Number of pages7
JournalPediatric Research
Volume63
Issue number6
DOIs
StatePublished - Jun 1 2008

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Cardiopulmonary Bypass
Biomarkers
Pediatrics
Electrophoresis
Proteins
Gels
Hemopexin
Pathology
Inflammation
alpha-Macroglobulins
Ceruloplasmin
Proteome
Epinephrine
Copper
Mass Spectrometry
Serotonin
Norepinephrine
Protein Isoforms
Oxidative Stress
Lasers

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Lull, M. E., Carkaci-Salli, N., Freeman, W. M., Myers, J. L., Midgley, F. M., Thomas, N. J., ... Ündar, A. (2008). Plasma biomarkers in pediatric patients undergoing cardiopulmonary bypass. Pediatric Research, 63(6), 638-644. https://doi.org/10.1203/PDR.0b013e31816e391f

Plasma biomarkers in pediatric patients undergoing cardiopulmonary bypass. / Lull, Melinda E.; Carkaci-Salli, Nurgul; Freeman, Willard M.; Myers, John L.; Midgley, Frank M.; Thomas, Neal J.; Kimatian, Stephen J.; Vrana, Kent E.; Ündar, Akif.

In: Pediatric Research, Vol. 63, No. 6, 01.06.2008, p. 638-644.

Research output: Contribution to journalArticle

Lull, ME, Carkaci-Salli, N, Freeman, WM, Myers, JL, Midgley, FM, Thomas, NJ, Kimatian, SJ, Vrana, KE & Ündar, A 2008, 'Plasma biomarkers in pediatric patients undergoing cardiopulmonary bypass', Pediatric Research, vol. 63, no. 6, pp. 638-644. https://doi.org/10.1203/PDR.0b013e31816e391f
Lull ME, Carkaci-Salli N, Freeman WM, Myers JL, Midgley FM, Thomas NJ et al. Plasma biomarkers in pediatric patients undergoing cardiopulmonary bypass. Pediatric Research. 2008 Jun 1;63(6):638-644. https://doi.org/10.1203/PDR.0b013e31816e391f
Lull, Melinda E. ; Carkaci-Salli, Nurgul ; Freeman, Willard M. ; Myers, John L. ; Midgley, Frank M. ; Thomas, Neal J. ; Kimatian, Stephen J. ; Vrana, Kent E. ; Ündar, Akif. / Plasma biomarkers in pediatric patients undergoing cardiopulmonary bypass. In: Pediatric Research. 2008 ; Vol. 63, No. 6. pp. 638-644.
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