Plasma hippurate in renal failure: High-performance liquid chromatography method and clinical application

P. Igarashi, P. Gulyassy, L. Stanfel, T. Depner

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We have developed a high-performance liquid chromatography (HPLC) method for assay of hippurate in plasma of patients with renal failure. Hippurate accounts, in part, for the impaired binding of drugs and metabolites to albumin and may cause other disorders in azotemic patients. The method is precise, accurate and reproducible. Among 25 patients with acute and chronic renal failure having serum creatinine in the range of 2.9-43 mg/dl (256-3,801 μmol/l), plasma hippurate ranged from 0.11 to 16.2 mg/dl (6.1-904 μmol/l). Hippurate concentration correlated moderately closely with plasma creatinine, urea and anion gap. Its curvilinear relation to the reciprocal of serum creatinine indicated a proportional decline of GFR and tubular function or the accumulation of inhibitors of the proximal tubular anion secretory pathway. The method should be useful for further studies of abnormal albumin binding as well as other disorders in azotemic patients.

Original languageEnglish (US)
Pages (from-to)290-294
Number of pages5
JournalNephron
Volume47
Issue number4
StatePublished - 1987

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Renal Insufficiency
High Pressure Liquid Chromatography
Creatinine
Albumins
Acid-Base Equilibrium
Secretory Pathway
Serum
Acute Kidney Injury
Chronic Kidney Failure
Anions
Urea
hippuric acid
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Nephrology

Cite this

Igarashi, P., Gulyassy, P., Stanfel, L., & Depner, T. (1987). Plasma hippurate in renal failure: High-performance liquid chromatography method and clinical application. Nephron, 47(4), 290-294.

Plasma hippurate in renal failure : High-performance liquid chromatography method and clinical application. / Igarashi, P.; Gulyassy, P.; Stanfel, L.; Depner, T.

In: Nephron, Vol. 47, No. 4, 1987, p. 290-294.

Research output: Contribution to journalArticle

Igarashi, P, Gulyassy, P, Stanfel, L & Depner, T 1987, 'Plasma hippurate in renal failure: High-performance liquid chromatography method and clinical application', Nephron, vol. 47, no. 4, pp. 290-294.
Igarashi, P. ; Gulyassy, P. ; Stanfel, L. ; Depner, T. / Plasma hippurate in renal failure : High-performance liquid chromatography method and clinical application. In: Nephron. 1987 ; Vol. 47, No. 4. pp. 290-294.
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