Plasma membrane calcium channels in human carcinoma A431 cells are functionally coupled to inositol 1,4,5-trisphosphate receptor- phosphatidylinositol 4,5-bisphosphate complexes

Elena Kaznacheyeva, Alexander Zubov, Anton Nikolaev, Vadim Alexeenko, Ilya Bezprozvanny, Galina N. Mozhayeva

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37 Scopus citations


In most nonexcitable cells, calcium (Ca2+) release from inositol 1,4,5-trisphosphate (InsP3)-sensitive intracellular Ca2+ stores is coupled to Ca2+ influx (calcium release-activated channels (I(CRAC))) pathway. Despite intense investigation, the molecular identity of I(CRAC) and the mechanism of its activation remain poorly understood. InsP3-dependent miniature calcium channels (I(min)) display functional properties characteristic for I(CRAC). Here we used patch clamp recordings of I(min) channels in human carcinoma A431 cells to demonstrate that I(min) activity was greatly enhanced in the presence of anti-phosphatidylinositol 4,5- bisphosphate antibody (PIP2Ab) and diminished in the presence of PIP2. AntiPIP2 antibody induced a greater than 6-fold increase in I(min) sensitivity for InsP3 activation and an almost 4-fold change in I(min) maximal open probability. The addition of exogenous PIP2 vesicles to the cytosolic surface of inside-out patches inhibited I(min) activity. These results lead us to propose an existence of a Ca2+ influx pathway in nonexcitable cells activated via direct conformational coupling with a selected population of InsP3 receptors, located just underneath the plasma membrane and coupled to PIP2. The described pathway provides for a highly compartmentalized Ca2+ influx and intracellular Ca2+ store refilling mechanism.

Original languageEnglish (US)
Pages (from-to)4561-4564
Number of pages4
JournalJournal of Biological Chemistry
Issue number7
StatePublished - Feb 18 2000


ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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