Plasmid pWW115, a cloning vector for use with Moraxella catarrhalis

Wei Wang, Eric J. Hansen

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The plasmid shuttle vector pWW102B is able to replicate in only a modest number of Moraxella catarrhalis strains. Plasmid pWW115, a spontaneous deletion mutant of pWW102B, was shown to lack both the pACYC184-derived origin of replication and the associated chloramphenicol-resistance gene but was able to replicate in every M. catarrhalis strain tested in this study, including one strain that had been previously refractory to all types of genetic manipulations. To test the utility of this plasmid, a M. catarrhalis gene encoding the UspA2 serum-resistance factor was cloned into pWW115 and the resultant recombinant plasmid was shown to confer serum-resistance on a serum-sensitive M. catarrhalis uspA2 mutant.

Original languageEnglish (US)
Pages (from-to)133-137
Number of pages5
JournalPlasmid
Volume56
Issue number2
DOIs
StatePublished - Sep 2006

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Moraxella (Branhamella) catarrhalis
Genetic Vectors
Plasmids
Serum
Chloramphenicol Resistance
Replication Origin
R Factors
Genes

Keywords

  • Complementation
  • Moraxella catarrhalis
  • Plasmid

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Plasmid pWW115, a cloning vector for use with Moraxella catarrhalis. / Wang, Wei; Hansen, Eric J.

In: Plasmid, Vol. 56, No. 2, 09.2006, p. 133-137.

Research output: Contribution to journalArticle

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