Platelet depletion in mice increases mortality after thermal injury

Satoshi Fujimi, Malcolm P. MacConmara, Adrian A. Maung, Yan Zang, John A. Mannick, James A. Lederer, Peter H. Lapchak

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Platelets play a fundamental role in maintaining hemostasis and have been shown to participate in innate and adaptive immunity. However, the role of platelets in the immune response to injury remains undefined. We tested the importance of platelets in the host response to serious injury in a newly developed platelet-deficient mouse model. Wild-type and platelet-depleted C57BL/6J mice underwent a 25% full-thickness total body surface area thermal or sham injury. Platelet-deficient mice showed survival of 51% at 48 hours after injury compared with 94% to 100% survival in experimental control mice (P < .001). Necropsy and histology ruled out hemorrhage and hypovolemia as causes of death. Percentages of peripheral blood monocytes (P < .01) and neutrophils (P < .05) were increased between 36 and 48 hours after thermal injury in platelet-deficient mice compared with control mice. Plasma levels of TNFα (P < .001), IL-6 (P < .001), and MCP-1 (P < .05) were also elevated by 24 hours whereas levels of TGFβ1 were reduced between 24 and 36 hours following injury in platelet-depleted mice (P < .001) compared with control mice. Our findings demonstrate for the first time that platelets play a critical protective role during the host response to injury. Moreover, our findings suggest that platelets and, more importantly, platelet-derived TGFβ1 modulate the systemic inflammatory response occurring after injury.

Original languageEnglish (US)
Pages (from-to)4399-4406
Number of pages8
JournalBlood
Volume107
Issue number11
DOIs
StatePublished - Jun 1 2006

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Platelets
Blood Platelets
Hot Temperature
Mortality
Wounds and Injuries
Hypovolemia
Histology
Body Surface Area
Adaptive Immunity
Hemostasis
Inbred C57BL Mouse
Innate Immunity
Monocytes
Cause of Death
Interleukin-6
Neutrophils
Blood
Hemorrhage
Plasmas

ASJC Scopus subject areas

  • Hematology

Cite this

Fujimi, S., MacConmara, M. P., Maung, A. A., Zang, Y., Mannick, J. A., Lederer, J. A., & Lapchak, P. H. (2006). Platelet depletion in mice increases mortality after thermal injury. Blood, 107(11), 4399-4406. https://doi.org/10.1182/blood-2005-09-3776

Platelet depletion in mice increases mortality after thermal injury. / Fujimi, Satoshi; MacConmara, Malcolm P.; Maung, Adrian A.; Zang, Yan; Mannick, John A.; Lederer, James A.; Lapchak, Peter H.

In: Blood, Vol. 107, No. 11, 01.06.2006, p. 4399-4406.

Research output: Contribution to journalArticle

Fujimi, S, MacConmara, MP, Maung, AA, Zang, Y, Mannick, JA, Lederer, JA & Lapchak, PH 2006, 'Platelet depletion in mice increases mortality after thermal injury', Blood, vol. 107, no. 11, pp. 4399-4406. https://doi.org/10.1182/blood-2005-09-3776
Fujimi S, MacConmara MP, Maung AA, Zang Y, Mannick JA, Lederer JA et al. Platelet depletion in mice increases mortality after thermal injury. Blood. 2006 Jun 1;107(11):4399-4406. https://doi.org/10.1182/blood-2005-09-3776
Fujimi, Satoshi ; MacConmara, Malcolm P. ; Maung, Adrian A. ; Zang, Yan ; Mannick, John A. ; Lederer, James A. ; Lapchak, Peter H. / Platelet depletion in mice increases mortality after thermal injury. In: Blood. 2006 ; Vol. 107, No. 11. pp. 4399-4406.
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