Platelet-derived growth factor mediates tyrosine phosphorylation of the cytoplasmic domain of the low density lipoprotein receptor-related protein in caveolae

Philippe Boucher, Pingsheng Liu, Michael Gotthardt, Thomas Hiesberger, Richard G W Anderson, Joachim Herz

Research output: Contribution to journalArticle

163 Citations (Scopus)

Abstract

The low density lipoprotein (LDL) receptor gene family represents a class of multifunctional, endocytic cell surface receptors. Recently, roles in cellular signaling have also emerged. For instance, the very low density lipoprotein receptor (VLDLR) and the apolipoprotein receptor-2 (apoER2) function in a developmental signaling pathway that regulates the lamination of cortical layers in the brain and involves the activation of tyrosine kinases. Furthermore, the cytoplasmic domain of the LDL receptor-related protein (LRP) was found to be a substrate for the non-receptor tyrosine kinase Src, but the physiological significance of this phosphorylation event remained unknown. Here we show that tyrosine phosphorylation of LRP occurs in caveolae and involves the platelet-derived growth factor (PDGF) receptor β and phosphoinositide 3-kinase. Receptor-associated protein, an antagonist of ligand binding to LRP, and apoE-enriched β-VLDL, a ligand for LRP, reduce PDGF-induced tyrosine phosphorylation of the LRP cytoplasmic domain. In the accompanying paper (Loukinova, E., Ranganathan, S., Kuznetsov, S., Gorlatova, N., Migliorini, M., Ulery, P. G., Mikhailenko, I., Lawrence, D. L., and Strickland, D. K. (2002) J. Biol. Chem. 277, 15499-15506) Loukinova et aL further demonstrate that one form of PDGF, PDGF-BB, binds specifically to LRP and that phosphorylation of LRP requires the activation of Src family kinases. Taken together, these findings provide a biochemical basis for a cellular signaling pathway that involves apoE and LRP.

Original languageEnglish (US)
Pages (from-to)15507-15513
Number of pages7
JournalJournal of Biological Chemistry
Volume277
Issue number18
DOIs
StatePublished - May 3 2002

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LDL-Receptor Related Proteins
Low Density Lipoprotein Receptor-Related Protein-1
Caveolae
Phosphorylation
LDL Receptors
Platelet-Derived Growth Factor
Tyrosine
Proteins
Cell signaling
src-Family Kinases
Apolipoproteins E
Chemical activation
Ligands
Platelet-Derived Growth Factor Receptors
1-Phosphatidylinositol 4-Kinase
Apolipoproteins
Cell Surface Receptors
Phosphatidylinositols
Protein-Tyrosine Kinases
Brain

ASJC Scopus subject areas

  • Biochemistry

Cite this

Platelet-derived growth factor mediates tyrosine phosphorylation of the cytoplasmic domain of the low density lipoprotein receptor-related protein in caveolae. / Boucher, Philippe; Liu, Pingsheng; Gotthardt, Michael; Hiesberger, Thomas; Anderson, Richard G W; Herz, Joachim.

In: Journal of Biological Chemistry, Vol. 277, No. 18, 03.05.2002, p. 15507-15513.

Research output: Contribution to journalArticle

Boucher, Philippe ; Liu, Pingsheng ; Gotthardt, Michael ; Hiesberger, Thomas ; Anderson, Richard G W ; Herz, Joachim. / Platelet-derived growth factor mediates tyrosine phosphorylation of the cytoplasmic domain of the low density lipoprotein receptor-related protein in caveolae. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 18. pp. 15507-15513.
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