Platelet storage under in vitro condition is associated with calcium-dependent apoptosis-like lesions and novel reorganization in platelet cytoskeleton

Vinita Wadhawan, Zubair A. Karim, Saikat Mukhopadhyay, Ramkrishna Gupta, Madhu Dikshit, Debabrata Dash

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Platelets are cleared from circulation after a life span of 8-10 days. The molecular mechanisms underlying platelet senescence remain poorly characterized. Here we report that, progressive functional impairment in the platelets incubated in vitro in a plasma-free isotonic medium for up to 24h at 37°C is associated with release of cytochrome c from platelet mitochondria and cleavage of procaspase-9, but without evidence of caspase-3 activation. Concomitantly, there was proteolysis of survival proteins like focal adhesion kinase, Src, gelsolin, and specific cytoskeleton-associated peptides, in a manner regulated by extracellular calcium and calpain activity. Cytoskeleton played a critical role as evidenced from the association of these proteins and their degradation products, as well as procaspase-3 and the actin regulatory small GTPase, CDC42Hs, with the cytoskeleton of the stored platelets. The cytoskeletal enrichment with specific proteins was not associated with increase in the content of F-actin and was cytochalasin-resistant, thus signifying a novel mechanism of interaction of the translocating proteins with the pre-existing cytoskeleton. There was progressive exposure of phosphatidylserine on the outer leaflet of platelet membrane and specific electron microscopic changes suggestive of apoptotic lesions. Based on these observations we discuss the caspase-independent but calpain-mediated signaling events in the stored platelets resembling the features of apoptosis in the nucleated cells.

Original languageEnglish (US)
Pages (from-to)183-190
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume422
Issue number2
DOIs
StatePublished - Feb 15 2004

Fingerprint

Platelets
Cytoskeleton
Blood Platelets
Apoptosis
Calcium
Calpain
Caspase 3
Proteolysis
Actins
Proteins
Cytochalasins
Gelsolin
Focal Adhesion Protein-Tyrosine Kinases
Mitochondria
Monomeric GTP-Binding Proteins
Caspase 9
Phosphatidylserines
Caspases
Cytochromes c
In Vitro Techniques

Keywords

  • Apoptosis
  • Calpain
  • Caspases
  • Cytochrome c
  • Cytoskeleton
  • Platelet storage

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Platelet storage under in vitro condition is associated with calcium-dependent apoptosis-like lesions and novel reorganization in platelet cytoskeleton. / Wadhawan, Vinita; Karim, Zubair A.; Mukhopadhyay, Saikat; Gupta, Ramkrishna; Dikshit, Madhu; Dash, Debabrata.

In: Archives of Biochemistry and Biophysics, Vol. 422, No. 2, 15.02.2004, p. 183-190.

Research output: Contribution to journalArticle

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