Platelets lacking PIP5KIγ have normal integrin activation but impaired cytoskeletal-membrane integrity and adhesion.

Yanfeng Wang, Liang Zhao, Aae Suzuki, Lurong Lian, Sang H. Min, Ziqian Wang, Rustem I. Litvinov, Timothy J. Stalker, Tadayuki Yago, Arkadiusz G. Klopocki, David W. Schmidtke, Helen Yin, John K. Choi, Rodger P. McEver, John W. Weisel, John H. Hartwig, Charles S. Abrams

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Three isoforms of phosphatidylinositol-4-phosphate 5-kinase (PIP5KIα, PIP5KIβ, and PIP5KIγ) can each catalyze the final step in the synthesis of phosphatidylinositol-4,5-bisphosphate (PIP2), which in turn can be either converted to second messengers or bind directly to and thereby regulate proteins such as talin. A widely quoted model speculates that only p90, a longer splice form of platelet-specific PIP5KIγ, but not the shorter p87 PIP5KIγ, regulates the ligand-binding activity of integrins via talin. However, when we used mice genetically engineered to lack only p90 PIP5KIγ, we found that p90 PIP5KIγ is not critical for integrin activation or platelet adhesion on collagen. However, p90 PIP5KIγ-null platelets do have impaired anchoring of their integrins to the underlying cytoskeleton. Platelets lacking both the p90 and p87 PIP5KIγ isoforms had normal integrin activation and actin dynamics, but impaired anchoring of their integrins to the cytoskeleton. Most importantly, they formed weak shear-resistant adhesions ex vivo and unstable vascular occlusions in vivo. Together, our studies demonstrate that, although PIP5KIγ is essential for normal platelet function, individual isoforms of PIP5KIγ fulfill unique roles for the integrin-dependent integrity of the membrane cytoskeleton and for the stabilization of platelet adhesion.

Original languageEnglish (US)
Pages (from-to)2743-2752
Number of pages10
JournalBlood
Volume121
Issue number14
DOIs
StatePublished - Apr 4 2013

Fingerprint

Platelets
Integrins
Blood Platelets
Adhesion
Chemical activation
Membranes
Cytoskeleton
Talin
Protein Isoforms
Platelet Activation
Second Messenger Systems
Phosphatidylinositols
Blood Vessels
Actins
Collagen
Stabilization
Ligands
Proteins

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

Wang, Y., Zhao, L., Suzuki, A., Lian, L., Min, S. H., Wang, Z., ... Abrams, C. S. (2013). Platelets lacking PIP5KIγ have normal integrin activation but impaired cytoskeletal-membrane integrity and adhesion. Blood, 121(14), 2743-2752. https://doi.org/10.1182/blood-2012-07-445205

Platelets lacking PIP5KIγ have normal integrin activation but impaired cytoskeletal-membrane integrity and adhesion. / Wang, Yanfeng; Zhao, Liang; Suzuki, Aae; Lian, Lurong; Min, Sang H.; Wang, Ziqian; Litvinov, Rustem I.; Stalker, Timothy J.; Yago, Tadayuki; Klopocki, Arkadiusz G.; Schmidtke, David W.; Yin, Helen; Choi, John K.; McEver, Rodger P.; Weisel, John W.; Hartwig, John H.; Abrams, Charles S.

In: Blood, Vol. 121, No. 14, 04.04.2013, p. 2743-2752.

Research output: Contribution to journalArticle

Wang, Y, Zhao, L, Suzuki, A, Lian, L, Min, SH, Wang, Z, Litvinov, RI, Stalker, TJ, Yago, T, Klopocki, AG, Schmidtke, DW, Yin, H, Choi, JK, McEver, RP, Weisel, JW, Hartwig, JH & Abrams, CS 2013, 'Platelets lacking PIP5KIγ have normal integrin activation but impaired cytoskeletal-membrane integrity and adhesion.', Blood, vol. 121, no. 14, pp. 2743-2752. https://doi.org/10.1182/blood-2012-07-445205
Wang, Yanfeng ; Zhao, Liang ; Suzuki, Aae ; Lian, Lurong ; Min, Sang H. ; Wang, Ziqian ; Litvinov, Rustem I. ; Stalker, Timothy J. ; Yago, Tadayuki ; Klopocki, Arkadiusz G. ; Schmidtke, David W. ; Yin, Helen ; Choi, John K. ; McEver, Rodger P. ; Weisel, John W. ; Hartwig, John H. ; Abrams, Charles S. / Platelets lacking PIP5KIγ have normal integrin activation but impaired cytoskeletal-membrane integrity and adhesion. In: Blood. 2013 ; Vol. 121, No. 14. pp. 2743-2752.
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AU - Zhao, Liang

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AU - Min, Sang H.

AU - Wang, Ziqian

AU - Litvinov, Rustem I.

AU - Stalker, Timothy J.

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AU - Klopocki, Arkadiusz G.

AU - Schmidtke, David W.

AU - Yin, Helen

AU - Choi, John K.

AU - McEver, Rodger P.

AU - Weisel, John W.

AU - Hartwig, John H.

AU - Abrams, Charles S.

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