Plexins are GTPase-activating proteins for Rap and are activated by induced dimerization

Yuxiao Wang, Huawei He, Nishi Srivastava, Sheikh Vikarunnessa, Yong Bin Chen, Jin Jiang, Christopher W. Cowan, Xuewu Zhang

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Plexins are cell surface receptors that bind to semaphorins and transduce signals that regulate neuronal development, immune responses, and other processes. Signaling through plexins has been proposed to rely on specific guanosine triphosphatase (GTPase)-activating protein (GAP) activity for R-Ras and M-Ras. Activation of this GAP activity of plexins appears to require simultaneous binding of semaphorin to the plexin extracellular domain and of the Rho GTPases Rac1 or Rnd1 to the cytoplasmic region. However, GAP activity of plexins has eluded detection in several recent studies. We show that the purified cytoplasmic region of plexin uses a noncanonical catalytic mechanism to act as a GAP for Rap, but not for R-Ras or M-Ras. The RapGAP activity of plexins was autoinhibited and was activated by induced dimerization. Biochemical and crystallographic analyses demonstrated that binding of Rho GTPases did not directly contribute to activation of plexin RapGAP activity. Semaphorin stimulated the RapGAP activity of full-length plexin in cells, which was required for plexin-mediated neuronal growth cone collapse. Together, these findings define a pathway for plexin signaling and provide insights into the mechanism for semaphorin-induced activation of plexins.

Original languageEnglish (US)
Article numberra6
JournalScience Signaling
Volume5
Issue number207
DOIs
StatePublished - Jan 17 2012

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Dimerization
Guanosine
Semaphorins
GTPase-Activating Proteins
rho GTP-Binding Proteins
Proteins
Chemical activation
Growth Cones
Cell Surface Receptors
plexin
Cones

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Plexins are GTPase-activating proteins for Rap and are activated by induced dimerization. / Wang, Yuxiao; He, Huawei; Srivastava, Nishi; Vikarunnessa, Sheikh; Chen, Yong Bin; Jiang, Jin; Cowan, Christopher W.; Zhang, Xuewu.

In: Science Signaling, Vol. 5, No. 207, ra6, 17.01.2012.

Research output: Contribution to journalArticle

Wang, Yuxiao ; He, Huawei ; Srivastava, Nishi ; Vikarunnessa, Sheikh ; Chen, Yong Bin ; Jiang, Jin ; Cowan, Christopher W. ; Zhang, Xuewu. / Plexins are GTPase-activating proteins for Rap and are activated by induced dimerization. In: Science Signaling. 2012 ; Vol. 5, No. 207.
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