Polo kinases regulate C. elegans embryonic polarity via binding to DYRK2-primed MEX-5 and MEX-6

Yuichi Nishi, Eric Rogers, Scott M. Robertson, Rueyling Lin

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Polo kinases are known key regulators of cell divisions. Here we report a novel, non-cell division function for polo kinases in embryonic polarity of newly fertilized Caenorhabditis elegans embryos. We show that polo kinases, via their polo box domains, bind to and regulate the activity of two key polarity proteins, MEX-5 and MEX-6. These polo kinases are asymmetrically localized along the anteroposterior axis of newly fertilized C. elegans embryos in a pattern identical to that of MEX-5 and MEX-6. This asymmetric localization of polo kinases depends on MEX-5 and MEX-6, as well as genes regulating MEX-5 and MEX-6 asymmetry. We identify an amino acid of MEX-5, T186, essential for polo binding and show that T186 is important for MEX-5 function in vivo. We also show that MBK-2, a developmentally regulated DYRK2 kinase activated at meiosis II, primes T186 for subsequent polo kinase-dependent phosphorylation. Prior phosphorylation of MEX-5 at T186 greatly enhances phosphorylation of MEX-5 by polo kinases in vitro. Our results provide a mechanism by which MEX-5 and MEX-6 function is temporally regulated during the crucial oocyte-to-embryo transition.

Original languageEnglish (US)
Pages (from-to)687-697
Number of pages11
JournalDevelopment
Volume135
Issue number4
DOIs
StatePublished - Feb 2008

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Phosphotransferases
Embryonic Structures
Phosphorylation
Caenorhabditis elegans
Meiosis
Cell Division
Oocytes
Amino Acids
Genes
Proteins

Keywords

  • Caenorhabditis elegans
  • Embryo
  • MEX-5
  • Polarity
  • Polo kinase

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Polo kinases regulate C. elegans embryonic polarity via binding to DYRK2-primed MEX-5 and MEX-6. / Nishi, Yuichi; Rogers, Eric; Robertson, Scott M.; Lin, Rueyling.

In: Development, Vol. 135, No. 4, 02.2008, p. 687-697.

Research output: Contribution to journalArticle

Nishi, Yuichi ; Rogers, Eric ; Robertson, Scott M. ; Lin, Rueyling. / Polo kinases regulate C. elegans embryonic polarity via binding to DYRK2-primed MEX-5 and MEX-6. In: Development. 2008 ; Vol. 135, No. 4. pp. 687-697.
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