Poly(ϵ-Caprolactone) Nanofiber Wrap Improves Nerve Regeneration and Functional Outcomes after Delayed Nerve Repair

Joseph Lopez, Kevin Xin, Amy Quan, Sinan Xiang, Angelo A.Leto Barone, Joshua Budihardjo, Leila Musavi, Sara Mulla, Richard Redett, Russell Martin, Hai Quan Mao, W. P. Andrew Lee, Zuhaib Ibrahim, Gerald Brandacher

Research output: Contribution to journalArticle

Abstract

Background: The purpose of this study was to assess the efficacy of biodegradable, electrospun poly(ϵ-caprolactone) nanofiber nerve conduits in improving nerve regeneration. Methods: The authors used a rat forelimb chronic denervation model to assess the effects of poly(ϵ-caprolactone) conduits on improving nerve regeneration and upper extremity function. Three groups of rats were examined: (1) negative-control animals (n = 5), which underwent 8 weeks of median nerve chronic denervation injury followed by repair with no conduit; (2) experimental animals (n = 5), which underwent 8 weeks of median nerve chronic denervation followed by repair and poly(ϵ-caprolactone) nerve conduit wrapping of the nerve coaptation site; and (3) positive-control animals (n = 5), which were naive controls. All animals underwent compound muscle action potential and functional testing. At 14 weeks after repair, the median nerve and flexor muscles were harvested for histologic analysis. Results: Histomorphometric analysis of regenerating median nerves demonstrated augmented axonal regeneration in experimental versus negative control animals (total axon count, 1769 ± 672 versus 1072 ± 123.80; p = 0.0468). With regard to functional recovery, experimental and negative-control animals (1.67 ± 0.04 versus 0.97 ± 0.39; p = 0.036) had regained 34.9 percent and 25.4 percent, respectively, of baseline hand grip strength at 14 weeks after repair. Lastly, less collagen deposition at the nerve coaptation site of experimental animals was found when compared to control animals (p < 0.05). Conclusion: Biodegradable, poly(ϵ-caprolactone) nanofiber nerve conduits can improve nerve regeneration and subsequent physiologic extremity function in the setting of delayed nerve repair by decreasing the scar burden at nerve coaptation sites.

Original languageEnglish (US)
Pages (from-to)48e-57e
JournalPlastic and reconstructive surgery
Volume144
Issue number1
DOIs
StatePublished - Jul 1 2019
Externally publishedYes

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Nanofibers
Nerve Regeneration
Median Nerve
Denervation
Hand Strength
Muscles
Forelimb
polycaprolactone
Upper Extremity
Action Potentials
Cicatrix
Axons
Regeneration
Collagen
Extremities
Wounds and Injuries

ASJC Scopus subject areas

  • Surgery

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Poly(ϵ-Caprolactone) Nanofiber Wrap Improves Nerve Regeneration and Functional Outcomes after Delayed Nerve Repair. / Lopez, Joseph; Xin, Kevin; Quan, Amy; Xiang, Sinan; Barone, Angelo A.Leto; Budihardjo, Joshua; Musavi, Leila; Mulla, Sara; Redett, Richard; Martin, Russell; Mao, Hai Quan; Andrew Lee, W. P.; Ibrahim, Zuhaib; Brandacher, Gerald.

In: Plastic and reconstructive surgery, Vol. 144, No. 1, 01.07.2019, p. 48e-57e.

Research output: Contribution to journalArticle

Lopez, J, Xin, K, Quan, A, Xiang, S, Barone, AAL, Budihardjo, J, Musavi, L, Mulla, S, Redett, R, Martin, R, Mao, HQ, Andrew Lee, WP, Ibrahim, Z & Brandacher, G 2019, 'Poly(ϵ-Caprolactone) Nanofiber Wrap Improves Nerve Regeneration and Functional Outcomes after Delayed Nerve Repair', Plastic and reconstructive surgery, vol. 144, no. 1, pp. 48e-57e. https://doi.org/10.1097/PRS.0000000000005715
Lopez, Joseph ; Xin, Kevin ; Quan, Amy ; Xiang, Sinan ; Barone, Angelo A.Leto ; Budihardjo, Joshua ; Musavi, Leila ; Mulla, Sara ; Redett, Richard ; Martin, Russell ; Mao, Hai Quan ; Andrew Lee, W. P. ; Ibrahim, Zuhaib ; Brandacher, Gerald. / Poly(ϵ-Caprolactone) Nanofiber Wrap Improves Nerve Regeneration and Functional Outcomes after Delayed Nerve Repair. In: Plastic and reconstructive surgery. 2019 ; Vol. 144, No. 1. pp. 48e-57e.
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abstract = "Background: The purpose of this study was to assess the efficacy of biodegradable, electrospun poly(ϵ-caprolactone) nanofiber nerve conduits in improving nerve regeneration. Methods: The authors used a rat forelimb chronic denervation model to assess the effects of poly(ϵ-caprolactone) conduits on improving nerve regeneration and upper extremity function. Three groups of rats were examined: (1) negative-control animals (n = 5), which underwent 8 weeks of median nerve chronic denervation injury followed by repair with no conduit; (2) experimental animals (n = 5), which underwent 8 weeks of median nerve chronic denervation followed by repair and poly(ϵ-caprolactone) nerve conduit wrapping of the nerve coaptation site; and (3) positive-control animals (n = 5), which were naive controls. All animals underwent compound muscle action potential and functional testing. At 14 weeks after repair, the median nerve and flexor muscles were harvested for histologic analysis. Results: Histomorphometric analysis of regenerating median nerves demonstrated augmented axonal regeneration in experimental versus negative control animals (total axon count, 1769 ± 672 versus 1072 ± 123.80; p = 0.0468). With regard to functional recovery, experimental and negative-control animals (1.67 ± 0.04 versus 0.97 ± 0.39; p = 0.036) had regained 34.9 percent and 25.4 percent, respectively, of baseline hand grip strength at 14 weeks after repair. Lastly, less collagen deposition at the nerve coaptation site of experimental animals was found when compared to control animals (p < 0.05). Conclusion: Biodegradable, poly(ϵ-caprolactone) nanofiber nerve conduits can improve nerve regeneration and subsequent physiologic extremity function in the setting of delayed nerve repair by decreasing the scar burden at nerve coaptation sites.",
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T1 - Poly(ϵ-Caprolactone) Nanofiber Wrap Improves Nerve Regeneration and Functional Outcomes after Delayed Nerve Repair

AU - Lopez, Joseph

AU - Xin, Kevin

AU - Quan, Amy

AU - Xiang, Sinan

AU - Barone, Angelo A.Leto

AU - Budihardjo, Joshua

AU - Musavi, Leila

AU - Mulla, Sara

AU - Redett, Richard

AU - Martin, Russell

AU - Mao, Hai Quan

AU - Andrew Lee, W. P.

AU - Ibrahim, Zuhaib

AU - Brandacher, Gerald

PY - 2019/7/1

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N2 - Background: The purpose of this study was to assess the efficacy of biodegradable, electrospun poly(ϵ-caprolactone) nanofiber nerve conduits in improving nerve regeneration. Methods: The authors used a rat forelimb chronic denervation model to assess the effects of poly(ϵ-caprolactone) conduits on improving nerve regeneration and upper extremity function. Three groups of rats were examined: (1) negative-control animals (n = 5), which underwent 8 weeks of median nerve chronic denervation injury followed by repair with no conduit; (2) experimental animals (n = 5), which underwent 8 weeks of median nerve chronic denervation followed by repair and poly(ϵ-caprolactone) nerve conduit wrapping of the nerve coaptation site; and (3) positive-control animals (n = 5), which were naive controls. All animals underwent compound muscle action potential and functional testing. At 14 weeks after repair, the median nerve and flexor muscles were harvested for histologic analysis. Results: Histomorphometric analysis of regenerating median nerves demonstrated augmented axonal regeneration in experimental versus negative control animals (total axon count, 1769 ± 672 versus 1072 ± 123.80; p = 0.0468). With regard to functional recovery, experimental and negative-control animals (1.67 ± 0.04 versus 0.97 ± 0.39; p = 0.036) had regained 34.9 percent and 25.4 percent, respectively, of baseline hand grip strength at 14 weeks after repair. Lastly, less collagen deposition at the nerve coaptation site of experimental animals was found when compared to control animals (p < 0.05). Conclusion: Biodegradable, poly(ϵ-caprolactone) nanofiber nerve conduits can improve nerve regeneration and subsequent physiologic extremity function in the setting of delayed nerve repair by decreasing the scar burden at nerve coaptation sites.

AB - Background: The purpose of this study was to assess the efficacy of biodegradable, electrospun poly(ϵ-caprolactone) nanofiber nerve conduits in improving nerve regeneration. Methods: The authors used a rat forelimb chronic denervation model to assess the effects of poly(ϵ-caprolactone) conduits on improving nerve regeneration and upper extremity function. Three groups of rats were examined: (1) negative-control animals (n = 5), which underwent 8 weeks of median nerve chronic denervation injury followed by repair with no conduit; (2) experimental animals (n = 5), which underwent 8 weeks of median nerve chronic denervation followed by repair and poly(ϵ-caprolactone) nerve conduit wrapping of the nerve coaptation site; and (3) positive-control animals (n = 5), which were naive controls. All animals underwent compound muscle action potential and functional testing. At 14 weeks after repair, the median nerve and flexor muscles were harvested for histologic analysis. Results: Histomorphometric analysis of regenerating median nerves demonstrated augmented axonal regeneration in experimental versus negative control animals (total axon count, 1769 ± 672 versus 1072 ± 123.80; p = 0.0468). With regard to functional recovery, experimental and negative-control animals (1.67 ± 0.04 versus 0.97 ± 0.39; p = 0.036) had regained 34.9 percent and 25.4 percent, respectively, of baseline hand grip strength at 14 weeks after repair. Lastly, less collagen deposition at the nerve coaptation site of experimental animals was found when compared to control animals (p < 0.05). Conclusion: Biodegradable, poly(ϵ-caprolactone) nanofiber nerve conduits can improve nerve regeneration and subsequent physiologic extremity function in the setting of delayed nerve repair by decreasing the scar burden at nerve coaptation sites.

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