Poly(A) nuclease interacts with the C-terminal domain of polyadenylate-binding protein domain from poly(A)-binding protein

Nadeem Siddiqui, David A. Mangus, Tsung Cheng Chang, Jeanne Marie Palermino, Ann Bin Shyu, Kalle Gehring

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The poly(A)-binding protein (PABP) is an essential protein found in all eukaryotes and is involved in an extensive range of cellular functions, including translation, mRNA metabolism, and mRNA export. Its C-terminal region contains a peptide-interacting PABC domain that recruits proteins containing a highly specific PAM-2 sequence motif to the messenger ribonucleoprotein complex. In humans, these proteins, including Paip1, Paip2, eRF3 (eukaryotic release factor 3), Ataxin-2, and Tob2, are all found to regulate translation through varying mechanisms. The following reports poly(A) nuclease (PAN) as a PABC-interacting partner in both yeast and humans. Their interaction is mediated by a PAM-2 motif identified within the PAN3 subunit. This site was identified in various fungal and animal species suggesting that the interaction is conserved throughout evolution. Our results indicate that PABP is directly involved in recruiting a deadenylase to the messenger ribonucleoprotein complex. This demonstrates a novel role for the PABC domain in mRNA metabolic processes and gives further insight into the function of PABP in mRNA maturation, export, and turnover.

Original languageEnglish (US)
Pages (from-to)25067-25075
Number of pages9
JournalJournal of Biological Chemistry
Volume282
Issue number34
DOIs
StatePublished - Aug 24 2007

Fingerprint

Poly(A)-Binding Proteins
Carrier Proteins
Messenger RNA
Protein Biosynthesis
Eukaryota
Proteins
Yeasts
Metabolism
Yeast
Peptides
Animals
poly(A)-specific ribonuclease
Protein Domains
pralidoxime
messenger ribonucleoprotein

ASJC Scopus subject areas

  • Biochemistry

Cite this

Poly(A) nuclease interacts with the C-terminal domain of polyadenylate-binding protein domain from poly(A)-binding protein. / Siddiqui, Nadeem; Mangus, David A.; Chang, Tsung Cheng; Palermino, Jeanne Marie; Shyu, Ann Bin; Gehring, Kalle.

In: Journal of Biological Chemistry, Vol. 282, No. 34, 24.08.2007, p. 25067-25075.

Research output: Contribution to journalArticle

Siddiqui, Nadeem ; Mangus, David A. ; Chang, Tsung Cheng ; Palermino, Jeanne Marie ; Shyu, Ann Bin ; Gehring, Kalle. / Poly(A) nuclease interacts with the C-terminal domain of polyadenylate-binding protein domain from poly(A)-binding protein. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 34. pp. 25067-25075.
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