Polyadenylated messenger RNA in paired helical filament-immunoreactive neurons in Alzheimer disease

W. S T Griffin; C. Ling; C. L. White; M. Morrison-Bogorad

doi:10.1097/00002093-199004020-00001

Polyadenylated messenger RNA in paired helical filament-immunoreactive neurons in Alzheimer disease

W. S T Griffin, C. Ling, C. L. White, M. Morrison-Bogorad

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

An antibody raised against isolated paired helical filaments (PHF) was used to identify tangle-bearing (PHF⁺) neurons in autopsy brain tissue from six Alzheimer disease (AD) patients and six age-matched controls (AMC). A comparison of the levels of polyadenylated messenger RNA [poly(A)⁺ mRNA] in PHF⁺ and PHF^- neurons of similar cross-sectional area in temporal and parietal lobe and cerebellum from four AD and four AMC brains was made by analysis of in situ hybridization of [³H] polyuridylate [poly(U)] to intracellular poly(A)⁺ mRNA. In PHF⁺ neurons, the level of poly(A)⁺ mRNA was approximately two-thirds that in similar-sized PHF^- neurons in either AD or AMC. The level of poly(A)⁺ mRNA in PHF^- neurons in regions of the brain that have more of the histopathologically defined effects, in AD was similar to that in regions with less effects.

Original language	English (US)
Pages (from-to)	69-78
Number of pages	10
Journal	Alzheimer disease and associated disorders
Volume	4
Issue number	2
DOIs	https://doi.org/10.1097/00002093-199004020-00001
State	Published - 1990

ASJC Scopus subject areas

Clinical Psychology
Gerontology
Geriatrics and Gerontology
Psychiatry and Mental health

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title = "Polyadenylated messenger RNA in paired helical filament-immunoreactive neurons in Alzheimer disease",

abstract = "An antibody raised against isolated paired helical filaments (PHF) was used to identify tangle-bearing (PHF+) neurons in autopsy brain tissue from six Alzheimer disease (AD) patients and six age-matched controls (AMC). A comparison of the levels of polyadenylated messenger RNA [poly(A)+ mRNA] in PHF+ and PHF- neurons of similar cross-sectional area in temporal and parietal lobe and cerebellum from four AD and four AMC brains was made by analysis of in situ hybridization of [3H] polyuridylate [poly(U)] to intracellular poly(A)+ mRNA. In PHF+ neurons, the level of poly(A)+ mRNA was approximately two-thirds that in similar-sized PHF- neurons in either AD or AMC. The level of poly(A)+ mRNA in PHF- neurons in regions of the brain that have more of the histopathologically defined effects, in AD was similar to that in regions with less effects.",

author = "Griffin, {W. S T} and C. Ling and White, {C. L.} and M. Morrison-Bogorad",

year = "1990",

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AU - Griffin, W. S T

AU - Ling, C.

AU - White, C. L.

AU - Morrison-Bogorad, M.

PY - 1990

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N2 - An antibody raised against isolated paired helical filaments (PHF) was used to identify tangle-bearing (PHF+) neurons in autopsy brain tissue from six Alzheimer disease (AD) patients and six age-matched controls (AMC). A comparison of the levels of polyadenylated messenger RNA [poly(A)+ mRNA] in PHF+ and PHF- neurons of similar cross-sectional area in temporal and parietal lobe and cerebellum from four AD and four AMC brains was made by analysis of in situ hybridization of [3H] polyuridylate [poly(U)] to intracellular poly(A)+ mRNA. In PHF+ neurons, the level of poly(A)+ mRNA was approximately two-thirds that in similar-sized PHF- neurons in either AD or AMC. The level of poly(A)+ mRNA in PHF- neurons in regions of the brain that have more of the histopathologically defined effects, in AD was similar to that in regions with less effects.

AB - An antibody raised against isolated paired helical filaments (PHF) was used to identify tangle-bearing (PHF+) neurons in autopsy brain tissue from six Alzheimer disease (AD) patients and six age-matched controls (AMC). A comparison of the levels of polyadenylated messenger RNA [poly(A)+ mRNA] in PHF+ and PHF- neurons of similar cross-sectional area in temporal and parietal lobe and cerebellum from four AD and four AMC brains was made by analysis of in situ hybridization of [3H] polyuridylate [poly(U)] to intracellular poly(A)+ mRNA. In PHF+ neurons, the level of poly(A)+ mRNA was approximately two-thirds that in similar-sized PHF- neurons in either AD or AMC. The level of poly(A)+ mRNA in PHF- neurons in regions of the brain that have more of the histopathologically defined effects, in AD was similar to that in regions with less effects.

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