Abstract
An antibody raised against isolated paired helical filaments (PHF) was used to identify tangle-bearing (PHF+) neurons in autopsy brain tissue from six Alzheimer disease (AD) patients and six age-matched controls (AMC). A comparison of the levels of polyadenylated messenger RNA [poly(A)+ mRNA] in PHF+ and PHF- neurons of similar cross-sectional area in temporal and parietal lobe and cerebellum from four AD and four AMC brains was made by analysis of in situ hybridization of [3H] polyuridylate [poly(U)] to intracellular poly(A)+ mRNA. In PHF+ neurons, the level of poly(A)+ mRNA was approximately two-thirds that in similar-sized PHF- neurons in either AD or AMC. The level of poly(A)+ mRNA in PHF- neurons in regions of the brain that have more of the histopathologically defined effects, in AD was similar to that in regions with less effects.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 69-78 |
| Number of pages | 10 |
| Journal | Alzheimer Disease and Associated Disorders |
| Volume | 4 |
| Issue number | 2 |
| State | Published - 1990 |
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ASJC Scopus subject areas
- Geriatrics and Gerontology
- Psychiatry and Mental health
- Neuroscience(all)
Cite this
Polyadenylated messenger RNA in paired helical filament-immunoreactive neurons in Alzheimer disease. / Griffin, W. S T; Ling, C.; White, C. L.; Morrison-Bogorad, M.
In: Alzheimer Disease and Associated Disorders, Vol. 4, No. 2, 1990, p. 69-78.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Polyadenylated messenger RNA in paired helical filament-immunoreactive neurons in Alzheimer disease
AU - Griffin, W. S T
AU - Ling, C.
AU - White, C. L.
AU - Morrison-Bogorad, M.
PY - 1990
Y1 - 1990
N2 - An antibody raised against isolated paired helical filaments (PHF) was used to identify tangle-bearing (PHF+) neurons in autopsy brain tissue from six Alzheimer disease (AD) patients and six age-matched controls (AMC). A comparison of the levels of polyadenylated messenger RNA [poly(A)+ mRNA] in PHF+ and PHF- neurons of similar cross-sectional area in temporal and parietal lobe and cerebellum from four AD and four AMC brains was made by analysis of in situ hybridization of [3H] polyuridylate [poly(U)] to intracellular poly(A)+ mRNA. In PHF+ neurons, the level of poly(A)+ mRNA was approximately two-thirds that in similar-sized PHF- neurons in either AD or AMC. The level of poly(A)+ mRNA in PHF- neurons in regions of the brain that have more of the histopathologically defined effects, in AD was similar to that in regions with less effects.
AB - An antibody raised against isolated paired helical filaments (PHF) was used to identify tangle-bearing (PHF+) neurons in autopsy brain tissue from six Alzheimer disease (AD) patients and six age-matched controls (AMC). A comparison of the levels of polyadenylated messenger RNA [poly(A)+ mRNA] in PHF+ and PHF- neurons of similar cross-sectional area in temporal and parietal lobe and cerebellum from four AD and four AMC brains was made by analysis of in situ hybridization of [3H] polyuridylate [poly(U)] to intracellular poly(A)+ mRNA. In PHF+ neurons, the level of poly(A)+ mRNA was approximately two-thirds that in similar-sized PHF- neurons in either AD or AMC. The level of poly(A)+ mRNA in PHF- neurons in regions of the brain that have more of the histopathologically defined effects, in AD was similar to that in regions with less effects.
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UR - http://www.scopus.com/inward/citedby.url?scp=0025143944&partnerID=8YFLogxK
M3 - Article
C2 - 1972628
AN - SCOPUS:0025143944
VL - 4
SP - 69
EP - 78
JO - Alzheimer Disease and Associated Disorders
JF - Alzheimer Disease and Associated Disorders
SN - 0893-0341
IS - 2
ER -