Polyadenylated messenger RNA in paired helical filament-immunoreactive neurons in Alzheimer disease

W. S T Griffin, C. Ling, C. L. White, M. Morrison-Bogorad

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

An antibody raised against isolated paired helical filaments (PHF) was used to identify tangle-bearing (PHF+) neurons in autopsy brain tissue from six Alzheimer disease (AD) patients and six age-matched controls (AMC). A comparison of the levels of polyadenylated messenger RNA [poly(A)+ mRNA] in PHF+ and PHF- neurons of similar cross-sectional area in temporal and parietal lobe and cerebellum from four AD and four AMC brains was made by analysis of in situ hybridization of [3H] polyuridylate [poly(U)] to intracellular poly(A)+ mRNA. In PHF+ neurons, the level of poly(A)+ mRNA was approximately two-thirds that in similar-sized PHF- neurons in either AD or AMC. The level of poly(A)+ mRNA in PHF- neurons in regions of the brain that have more of the histopathologically defined effects, in AD was similar to that in regions with less effects.

Original languageEnglish (US)
Pages (from-to)69-78
Number of pages10
JournalAlzheimer Disease and Associated Disorders
Volume4
Issue number2
StatePublished - 1990

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Alzheimer Disease
Neurons
Messenger RNA
Brain
Parietal Lobe
Temporal Lobe
Cerebellum
In Situ Hybridization
Autopsy
Antibodies

ASJC Scopus subject areas

  • Geriatrics and Gerontology
  • Psychiatry and Mental health
  • Neuroscience(all)

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Polyadenylated messenger RNA in paired helical filament-immunoreactive neurons in Alzheimer disease. / Griffin, W. S T; Ling, C.; White, C. L.; Morrison-Bogorad, M.

In: Alzheimer Disease and Associated Disorders, Vol. 4, No. 2, 1990, p. 69-78.

Research output: Contribution to journalArticle

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