Polybromo protein BAF180 functions in mammalian cardiac chamber maturation

Zhong Wang, Weiguo Zhai, James A. Richardson, Eric N. Olson, Juanito J. Meneses, Meri T. Firpo, Chulho Kang, William C. Skarnes, Robert Tjian

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

BAF and PBAF are two related mammalian chromatin remodeling complexes essential for gene expression and development. PBAF, but not BAF, is able to potentiate transcriptional activation in vitro mediated by nuclear receptors, such as RXRα, VDR, and PPARγ. Here we show that the ablation of PBAF-specific subunit BAF180 in mouse embryos results in severe hypoplastic ventricle development and trophoblast placental defects, similar to those found in mice lacking RXRα and PPARγ. Embryonic aggregation analyses reveal that in contrast to PPARγ-deficient mice, the heart defects are likely a direct result of BAF180 ablation, rather than an indirect consequence of trophoblast placental defects. We identified potential target genes for BAF180 in heart development, such as S100A13 as well as retinoic acid (RA)-induced targets RARβ2 and CRABPII. Importantly, BAF180 is recruited to the promoter of these target genes and BAF180 deficiency affects the RA response for CRABPII and RARβ2. These studies reveal unique functions of PBAF in cardiac chamber maturation.

Original languageEnglish (US)
Pages (from-to)3106-3116
Number of pages11
JournalGenes and Development
Volume18
Issue number24
DOIs
StatePublished - Dec 15 2004

Fingerprint

Peroxisome Proliferator-Activated Receptors
Trophoblasts
Tretinoin
Placentation
Proteins
Chromatin Assembly and Disassembly
Essential Genes
Cytoplasmic and Nuclear Receptors
Transcriptional Activation
Genes
Embryonic Structures
Gene Expression

Keywords

  • Chromatin remodeling
  • Heart
  • PBAF (SWI/SNF6b)
  • Placenta
  • Polybromo protein BAF180
  • Retinoic acid (RA) signaling

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Polybromo protein BAF180 functions in mammalian cardiac chamber maturation. / Wang, Zhong; Zhai, Weiguo; Richardson, James A.; Olson, Eric N.; Meneses, Juanito J.; Firpo, Meri T.; Kang, Chulho; Skarnes, William C.; Tjian, Robert.

In: Genes and Development, Vol. 18, No. 24, 15.12.2004, p. 3106-3116.

Research output: Contribution to journalArticle

Wang, Z, Zhai, W, Richardson, JA, Olson, EN, Meneses, JJ, Firpo, MT, Kang, C, Skarnes, WC & Tjian, R 2004, 'Polybromo protein BAF180 functions in mammalian cardiac chamber maturation', Genes and Development, vol. 18, no. 24, pp. 3106-3116. https://doi.org/10.1101/gad.1238104
Wang, Zhong ; Zhai, Weiguo ; Richardson, James A. ; Olson, Eric N. ; Meneses, Juanito J. ; Firpo, Meri T. ; Kang, Chulho ; Skarnes, William C. ; Tjian, Robert. / Polybromo protein BAF180 functions in mammalian cardiac chamber maturation. In: Genes and Development. 2004 ; Vol. 18, No. 24. pp. 3106-3116.
@article{e2cb964839d64be9a97921aeee92b7e9,
title = "Polybromo protein BAF180 functions in mammalian cardiac chamber maturation",
abstract = "BAF and PBAF are two related mammalian chromatin remodeling complexes essential for gene expression and development. PBAF, but not BAF, is able to potentiate transcriptional activation in vitro mediated by nuclear receptors, such as RXRα, VDR, and PPARγ. Here we show that the ablation of PBAF-specific subunit BAF180 in mouse embryos results in severe hypoplastic ventricle development and trophoblast placental defects, similar to those found in mice lacking RXRα and PPARγ. Embryonic aggregation analyses reveal that in contrast to PPARγ-deficient mice, the heart defects are likely a direct result of BAF180 ablation, rather than an indirect consequence of trophoblast placental defects. We identified potential target genes for BAF180 in heart development, such as S100A13 as well as retinoic acid (RA)-induced targets RARβ2 and CRABPII. Importantly, BAF180 is recruited to the promoter of these target genes and BAF180 deficiency affects the RA response for CRABPII and RARβ2. These studies reveal unique functions of PBAF in cardiac chamber maturation.",
keywords = "Chromatin remodeling, Heart, PBAF (SWI/SNF6b), Placenta, Polybromo protein BAF180, Retinoic acid (RA) signaling",
author = "Zhong Wang and Weiguo Zhai and Richardson, {James A.} and Olson, {Eric N.} and Meneses, {Juanito J.} and Firpo, {Meri T.} and Chulho Kang and Skarnes, {William C.} and Robert Tjian",
year = "2004",
month = "12",
day = "15",
doi = "10.1101/gad.1238104",
language = "English (US)",
volume = "18",
pages = "3106--3116",
journal = "Genes and Development",
issn = "0890-9369",
publisher = "Cold Spring Harbor Laboratory Press",
number = "24",

}

TY - JOUR

T1 - Polybromo protein BAF180 functions in mammalian cardiac chamber maturation

AU - Wang, Zhong

AU - Zhai, Weiguo

AU - Richardson, James A.

AU - Olson, Eric N.

AU - Meneses, Juanito J.

AU - Firpo, Meri T.

AU - Kang, Chulho

AU - Skarnes, William C.

AU - Tjian, Robert

PY - 2004/12/15

Y1 - 2004/12/15

N2 - BAF and PBAF are two related mammalian chromatin remodeling complexes essential for gene expression and development. PBAF, but not BAF, is able to potentiate transcriptional activation in vitro mediated by nuclear receptors, such as RXRα, VDR, and PPARγ. Here we show that the ablation of PBAF-specific subunit BAF180 in mouse embryos results in severe hypoplastic ventricle development and trophoblast placental defects, similar to those found in mice lacking RXRα and PPARγ. Embryonic aggregation analyses reveal that in contrast to PPARγ-deficient mice, the heart defects are likely a direct result of BAF180 ablation, rather than an indirect consequence of trophoblast placental defects. We identified potential target genes for BAF180 in heart development, such as S100A13 as well as retinoic acid (RA)-induced targets RARβ2 and CRABPII. Importantly, BAF180 is recruited to the promoter of these target genes and BAF180 deficiency affects the RA response for CRABPII and RARβ2. These studies reveal unique functions of PBAF in cardiac chamber maturation.

AB - BAF and PBAF are two related mammalian chromatin remodeling complexes essential for gene expression and development. PBAF, but not BAF, is able to potentiate transcriptional activation in vitro mediated by nuclear receptors, such as RXRα, VDR, and PPARγ. Here we show that the ablation of PBAF-specific subunit BAF180 in mouse embryos results in severe hypoplastic ventricle development and trophoblast placental defects, similar to those found in mice lacking RXRα and PPARγ. Embryonic aggregation analyses reveal that in contrast to PPARγ-deficient mice, the heart defects are likely a direct result of BAF180 ablation, rather than an indirect consequence of trophoblast placental defects. We identified potential target genes for BAF180 in heart development, such as S100A13 as well as retinoic acid (RA)-induced targets RARβ2 and CRABPII. Importantly, BAF180 is recruited to the promoter of these target genes and BAF180 deficiency affects the RA response for CRABPII and RARβ2. These studies reveal unique functions of PBAF in cardiac chamber maturation.

KW - Chromatin remodeling

KW - Heart

KW - PBAF (SWI/SNF6b)

KW - Placenta

KW - Polybromo protein BAF180

KW - Retinoic acid (RA) signaling

UR - http://www.scopus.com/inward/record.url?scp=10644277238&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=10644277238&partnerID=8YFLogxK

U2 - 10.1101/gad.1238104

DO - 10.1101/gad.1238104

M3 - Article

C2 - 15601824

AN - SCOPUS:10644277238

VL - 18

SP - 3106

EP - 3116

JO - Genes and Development

JF - Genes and Development

SN - 0890-9369

IS - 24

ER -