Polycation comb-type copolymer reduces counterion condensation effect to stabilize DNA duplex and triplex formation

Atsushi Maruyama, Yu Ichiro Ohnishi, Hiromitsu Watanabe, Hidetaka Torigoe, Anwarul Ferdous, Toshihiro Akaike

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We have previously demonstrated that the polycation comb-type copolymer having abundant grafts of hydrophilic polymer chains significantly stabilizes DNA duplexes and triplexes [Maruyama et al., Bioconjugate Chem., 8 (1997) 3, Ferdous et al., Nucleic Acids Res., 26 (1998) 39]. This study was designed to estimate the mechanisms involved in the copolymer-mediated stabilization of DNA duplexes and triplexes. The melting temperatures, T(m), of DNA duplex and triplex increased with increasing salt concentration, as well documented by the Poisson-Boltzmann and counterion condensation theories that were originally proposed by Manning [J. Chem. Phys., 51 (1969) 924] and further elaborated by Manning [Biopolymers 11 (1972) 937, Biopolymers. 15 (1976) 2385] and Record [Biopolymers, 14 (1975) 2137-2158, Biopolymers, 15 (1976) 893]. In the presence of the copolymer, however, the T(m) values of DNA duplexes and triplexes did not show significant change with salt concentration. It was concluded that the copolymer is capable of reducing the counterion condensation effects to stabilize DNA duplexes and triplexes. Strong but exchangeable interaction between the copolymer and DNA is seemingly involved in the stabilization behavior.

Original languageEnglish (US)
Pages (from-to)273-280
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume16
Issue number1-4
DOIs
StatePublished - Nov 1999

Fingerprint

Comb and Wattles
Biopolymers
Condensation
copolymers
DNA
Copolymers
deoxyribonucleic acid
condensation
biopolymers
Salts
Stabilization
stabilization
Nucleic Acids
Freezing
salts
Polymers
Nucleic acids
nucleic acids
Transplants
Grafts

Keywords

  • Counterion condensation
  • Dextran
  • Duplex DNA
  • Graft copolymer
  • Interpolyelectrolyte complex
  • Poly(L-lysine)
  • Triplex DNA

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Polycation comb-type copolymer reduces counterion condensation effect to stabilize DNA duplex and triplex formation. / Maruyama, Atsushi; Ohnishi, Yu Ichiro; Watanabe, Hiromitsu; Torigoe, Hidetaka; Ferdous, Anwarul; Akaike, Toshihiro.

In: Colloids and Surfaces B: Biointerfaces, Vol. 16, No. 1-4, 11.1999, p. 273-280.

Research output: Contribution to journalArticle

Maruyama, Atsushi ; Ohnishi, Yu Ichiro ; Watanabe, Hiromitsu ; Torigoe, Hidetaka ; Ferdous, Anwarul ; Akaike, Toshihiro. / Polycation comb-type copolymer reduces counterion condensation effect to stabilize DNA duplex and triplex formation. In: Colloids and Surfaces B: Biointerfaces. 1999 ; Vol. 16, No. 1-4. pp. 273-280.
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