Pooled human albumin primes neutrophils

Jennifer L. Rabaglia, Ricardo Gonzalez, Ernest E. Moore, Alden H. Harken

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Human pooled albumin has traditionally been used both to pacify the artificial surfaces in a cardiopulmonary bypass circuit and also for volume repletion following surgery. In evaluating the routine use of albumin in multiple phases of cardiac surgery, conscientious surgical teams must assess both the physiological and financial price of albumin. Albumin indiscriminately binds many plasma proteins and lipids. In this series of experiments, we explored the influence of highly purified albumin devoid of bound lipids and globulins on both receptor-dependent (FMLP) and receptor-independent (PMA) priming/activation of human neutrophils. We believe that it is important to distinguish the direct influence of albumin from the albumin-bound proteins and lipids. We, therefore, also examined the effect of clinically accessible human pooled albumin on human neutrophils. We observed a dose-dependent priming/activation (elastase release) of human neutrophils by both pooled and purified albumin. We conclude that it is increasingly difficult to justify the routine use of albumin in cardiac surgical patients.

Original languageEnglish (US)
Pages (from-to)209-213
Number of pages5
JournalJournal of Cardiac Surgery
Volume17
Issue number3
StatePublished - May 2002

Fingerprint

Albumins
Neutrophils
Lipids
Formyl Peptide Receptor
Neutrophil Activation
Pancreatic Elastase
Globulins
Cardiopulmonary Bypass
Thoracic Surgery
Blood Proteins

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Rabaglia, J. L., Gonzalez, R., Moore, E. E., & Harken, A. H. (2002). Pooled human albumin primes neutrophils. Journal of Cardiac Surgery, 17(3), 209-213.

Pooled human albumin primes neutrophils. / Rabaglia, Jennifer L.; Gonzalez, Ricardo; Moore, Ernest E.; Harken, Alden H.

In: Journal of Cardiac Surgery, Vol. 17, No. 3, 05.2002, p. 209-213.

Research output: Contribution to journalArticle

Rabaglia, JL, Gonzalez, R, Moore, EE & Harken, AH 2002, 'Pooled human albumin primes neutrophils', Journal of Cardiac Surgery, vol. 17, no. 3, pp. 209-213.
Rabaglia JL, Gonzalez R, Moore EE, Harken AH. Pooled human albumin primes neutrophils. Journal of Cardiac Surgery. 2002 May;17(3):209-213.
Rabaglia, Jennifer L. ; Gonzalez, Ricardo ; Moore, Ernest E. ; Harken, Alden H. / Pooled human albumin primes neutrophils. In: Journal of Cardiac Surgery. 2002 ; Vol. 17, No. 3. pp. 209-213.
@article{ce9889b88e574edea282597bdac82eab,
title = "Pooled human albumin primes neutrophils",
abstract = "Human pooled albumin has traditionally been used both to pacify the artificial surfaces in a cardiopulmonary bypass circuit and also for volume repletion following surgery. In evaluating the routine use of albumin in multiple phases of cardiac surgery, conscientious surgical teams must assess both the physiological and financial price of albumin. Albumin indiscriminately binds many plasma proteins and lipids. In this series of experiments, we explored the influence of highly purified albumin devoid of bound lipids and globulins on both receptor-dependent (FMLP) and receptor-independent (PMA) priming/activation of human neutrophils. We believe that it is important to distinguish the direct influence of albumin from the albumin-bound proteins and lipids. We, therefore, also examined the effect of clinically accessible human pooled albumin on human neutrophils. We observed a dose-dependent priming/activation (elastase release) of human neutrophils by both pooled and purified albumin. We conclude that it is increasingly difficult to justify the routine use of albumin in cardiac surgical patients.",
author = "Rabaglia, {Jennifer L.} and Ricardo Gonzalez and Moore, {Ernest E.} and Harken, {Alden H.}",
year = "2002",
month = "5",
language = "English (US)",
volume = "17",
pages = "209--213",
journal = "Journal of Cardiac Surgery",
issn = "0886-0440",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Pooled human albumin primes neutrophils

AU - Rabaglia, Jennifer L.

AU - Gonzalez, Ricardo

AU - Moore, Ernest E.

AU - Harken, Alden H.

PY - 2002/5

Y1 - 2002/5

N2 - Human pooled albumin has traditionally been used both to pacify the artificial surfaces in a cardiopulmonary bypass circuit and also for volume repletion following surgery. In evaluating the routine use of albumin in multiple phases of cardiac surgery, conscientious surgical teams must assess both the physiological and financial price of albumin. Albumin indiscriminately binds many plasma proteins and lipids. In this series of experiments, we explored the influence of highly purified albumin devoid of bound lipids and globulins on both receptor-dependent (FMLP) and receptor-independent (PMA) priming/activation of human neutrophils. We believe that it is important to distinguish the direct influence of albumin from the albumin-bound proteins and lipids. We, therefore, also examined the effect of clinically accessible human pooled albumin on human neutrophils. We observed a dose-dependent priming/activation (elastase release) of human neutrophils by both pooled and purified albumin. We conclude that it is increasingly difficult to justify the routine use of albumin in cardiac surgical patients.

AB - Human pooled albumin has traditionally been used both to pacify the artificial surfaces in a cardiopulmonary bypass circuit and also for volume repletion following surgery. In evaluating the routine use of albumin in multiple phases of cardiac surgery, conscientious surgical teams must assess both the physiological and financial price of albumin. Albumin indiscriminately binds many plasma proteins and lipids. In this series of experiments, we explored the influence of highly purified albumin devoid of bound lipids and globulins on both receptor-dependent (FMLP) and receptor-independent (PMA) priming/activation of human neutrophils. We believe that it is important to distinguish the direct influence of albumin from the albumin-bound proteins and lipids. We, therefore, also examined the effect of clinically accessible human pooled albumin on human neutrophils. We observed a dose-dependent priming/activation (elastase release) of human neutrophils by both pooled and purified albumin. We conclude that it is increasingly difficult to justify the routine use of albumin in cardiac surgical patients.

UR - http://www.scopus.com/inward/record.url?scp=0036589539&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036589539&partnerID=8YFLogxK

M3 - Article

C2 - 12489905

AN - SCOPUS:0036589539

VL - 17

SP - 209

EP - 213

JO - Journal of Cardiac Surgery

JF - Journal of Cardiac Surgery

SN - 0886-0440

IS - 3

ER -