Positive autoregulation of the Myocyte enhancer factor-2 myogenic control gene during somatic muscle development in Drosophila

Richard M. Cripps, TyAnna L. Lovato, Eric N. Olson

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

The myocyte enhancer factor-2 (MEF2) transcription factor plays a central role in the activation and maintenance of muscle gene expression in fruit flies and vertebrates. The mechanism of action and downstream target genes of MEF2 have been defined in considerable detail, but relatively little is known about the mechanisms that regulate MEF2 expression during muscle development. Here we demonstrate that MEF2 maintains its own expression in all differentiated muscle cell types during late embryonic and larval development in Drosophila by binding a conserved MEF2 site in a muscle-specific regulatory enhancer. Ectopic expression of Mef2 is sufficient to directly activate this enhancer in some, but not all, non-muscle cells. Furthermore, activation of the Mef2 enhancer normally in muscle cells and ectopically in non-muscle cells is dependent upon the integrity of the MEF2 binding site. These findings suggest an evolutionarily conserved mechanism whereby MEF2 can stabilize the muscle phenotype by sustaining its own expression through a myogenic autoregulatory loop.

Original languageEnglish (US)
Pages (from-to)536-547
Number of pages12
JournalDevelopmental Biology
Volume267
Issue number2
DOIs
StatePublished - Mar 15 2004

Keywords

  • Autoregulation
  • Drosophila
  • Enhancer
  • Evolutionary conservation
  • Mesoderm
  • Muscle
  • Myocyte enhancer factor-2
  • Transcription

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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