Positive selection by the pre-TCR yields mature CD8+ T cells

Yuriko Ito, Satoko Arai, Nicolai S C Van Oers, Iannis Aifantis, Harald Von Boehmer, Toru Miyazaki

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

It has been of much interest whether there is functional redundancy between the constitutively signaling pre-Tα/TCRβ (pre-TCR) and ligated TCRαβ complexes, which independently operate the two distinct checkpoints during thymocyte development, i.e., the pre-TCR involved in β-selection at the CD4-CD8- double-negative stage and the TCRαβ being crucial for positive/negative selection at the CD4+CD8+ double-positive stage. We found that the pre-TCR expressed on double-positive cells in TCRα-deficient (TCRα-/-) mice produced a small number of mature CD8+ T cells. Surprisingly, when pre-Tα was overexpressed, resulting in augmentation of pre-TCR expression, there was a striking increase of the CD8+ T cells. In addition, even in the absence of up-regulation of pre-TCR expression, a similar increase of CD8+ T cells was also observed in TCRα-/- mice overexpressing Egr-1, which lowers the threshold of signal strength required for positive selection. In sharp contrast, the CD8+ T cells drastically decreased in the absence of pre-Tα on a TCRα-/- background. Thus, the pre-TCR appears to functionally promote positive selection of CD8+ T cells. The biased production of CD8+ T cells via the pre-TCR might also support the potential involvement of signal strength in CD4/CD8 lineage commitment.

Original languageEnglish (US)
Pages (from-to)4913-4919
Number of pages7
JournalJournal of Immunology
Volume169
Issue number9
DOIs
StatePublished - Nov 1 2002

    Fingerprint

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Ito, Y., Arai, S., Van Oers, N. S. C., Aifantis, I., Von Boehmer, H., & Miyazaki, T. (2002). Positive selection by the pre-TCR yields mature CD8+ T cells. Journal of Immunology, 169(9), 4913-4919. https://doi.org/10.4049/jimmunol.169.9.4913