Possible integration of upstream signals at Cdc42 in filamentous differentiation of S. cerevisiae

Xiaofeng Wu, Yi Wei Jiang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Various environmental stimuli (such as nitrogen starvation, short-chain alcohols and slowed DNA synthesis) induce filamentous differentiation in S. cerevisiae. Genetic mutations (such as deletion of the mitotic cyclin gene CLB2) cause constitutive filamentous differentiation. Although different stimulus-induced filamentous differentiation involves different signalling pathways, Cdc42 has been identified as a common regulator. We show here that Cdc42 is also required for hydroxyurea (HU)-induced and clb2Δ-caused filamentous growth. We show that the mitotic CDK Clb2/Cdc28 functions upstream of Cdc42 in regulating filamentous differentiation. This result points to possible existence of a Cdc42-MAPK-Clb2/Cdc28 positive feedback loop in the signalling of filamentous differentiation. We report isolation of a cdc42-Y40F allele that blocks HU-induced, but not nitrogen starvation-induced, short-chain alcohol-induced or clb2Δ-caused, filamentation. Based on these results, we propose a model in which Cdc42 functions as a possible integrator for the upstream signals of filamentous differentiation (from the filamentous growth MAPK pathway, the cAMP pathway and the Mec1/Rad53 checkpoint pathway). We also show evidence that the mitotic CDK inhibitor Swe1 may mediate the cross-talk between the cAMP and MAPK pathways.

Original languageEnglish (US)
Pages (from-to)1069-1077
Number of pages9
JournalYeast
Volume22
Issue number13
DOIs
StatePublished - Oct 15 2005

Fingerprint

hydroxyurea
Hydroxyurea
Starvation
starvation
Saccharomyces cerevisiae
Alcohols
Nitrogen
alcohols
Cyclins
cyclins
nitrogen
Growth
DNA
Genes
Alleles
alleles
Feedback
mutation
Mutation
synthesis

Keywords

  • Cdc42
  • Clb2/Cdc28
  • Filamentous growth
  • MAPK
  • Mec1/Rad53
  • S. cerevisiae

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Microbiology

Cite this

Possible integration of upstream signals at Cdc42 in filamentous differentiation of S. cerevisiae. / Wu, Xiaofeng; Jiang, Yi Wei.

In: Yeast, Vol. 22, No. 13, 15.10.2005, p. 1069-1077.

Research output: Contribution to journalArticle

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