Post-exercise cold water immersion does not alter high intensity interval training-induced exercise performance and Hsp72 responses, but enhances mitochondrial markers

Paula Fernandes Aguiar, Sílvia Mourão Magalhães, Ivana Alice Teixeira Fonseca, Vanessa Batista da Costa Santos, Mariana Aguiar de Matos, Marco Fabrício Dias Peixoto, Fábio Yuzo Nakamura, Craig Crandall, Hygor Nunes Araújo, Leonardo Reis Silveira, Etel Rocha-Vieira, Flávio de Castro Magalhães, Fabiano Trigueiro Amorim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

This study aims to evaluate the effect of regular post-exercise cold water immersion (CWI) on intramuscular markers of cellular stress response and signaling molecules related to mitochondria biogenesis and exercise performance after 4 weeks of high intensity interval training (HIIT). Seventeen healthy subjects were allocated into two groups: control (CON, n = 9) or CWI (n = 8). Each HIIT session consisted of 8–12 cycling exercise stimuli (90–110 % of peak power) for 60 s followed by 75 s of active recovery three times per week, for 4 weeks (12 HIIT sessions). After each HIIT session, the CWI had their lower limbs immersed in cold water (10 °C) for 15 min and the CON recovered at room temperature. Exercise performance was evaluated before and after HIIT by a 15-km cycling time trial. Vastus lateralis biopsies were obtained pre and 72 h post training. Samples were analyzed for heat shock protein 72 kDa (Hsp72), adenosine monophosphate-activated protein kinase (AMPK), and phosphorylated p38 mitogen-activated protein kinase (p-p38 MAPK) assessed by western blot. In addition, the mRNA expression of heat shock factor-1 (HSF-1), peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α), nuclear respiratory factor 1 and 2 (NRF1 and 2), mitochondrial transcription factor A (Tfam), calcium calmodulin-dependent protein kinase 2 (CaMK2) and enzymes citrate synthase (CS), carnitine palmitoyltransferase I (CPT1), and pyruvate dehydrogenase kinase (PDK4) were assessed by real-time PCR. Time to complete the 15-km cycling time trial was reduced with training (p < 0.001), but was not different between groups (p = 0.33). The Hsp72 (p = 0.01), p38 MAPK, and AMPK (p = 0.04) contents increased with training, but were not different between groups (p > 0.05). No differences were observed with training or condition for mRNA expression of PGC-1α (p = 0.31), CPT1 (p = 0.14), CS (p = 0.44), and NRF-2 (p = 0.82). However, HFS-1 (p = 0.007), PDK4 (p = 0.03), and Tfam (p = 0.03) mRNA were higher in CWI. NRF-1 decrease in both groups after training (p = 0.006). CaMK2 decreased with HIIT (p = 0.003) but it was not affected by CWI (p = 0.99). Cold water immersion does not alter HIIT-induced Hsp72, AMPK, p38 MAPK, and exercise performance but was able to increase some markers of cellular stress response and signaling molecules related to mitochondria biogenesis.

Original languageEnglish (US)
Pages (from-to)793-804
Number of pages12
JournalCell Stress and Chaperones
Volume21
Issue number5
DOIs
StatePublished - Sep 1 2016

Fingerprint

Immersion
Heat-Shock Proteins
Exercise
Water
Citrate (si)-Synthase
Calcium-Calmodulin-Dependent Protein Kinases
Mitochondria
p38 Mitogen-Activated Protein Kinases
Adenosine Monophosphate
Protein Kinases
Messenger RNA
GA-Binding Protein Transcription Factor
Nuclear Respiratory Factor 1
Carnitine O-Palmitoyltransferase
Molecules
Biopsy
Quadriceps Muscle
High-Intensity Interval Training
Real-Time Polymerase Chain Reaction
Lower Extremity

Keywords

  • Cold water immersion
  • Heat shock protein
  • High intensity interval training
  • Mitochondria biogenesis
  • Post-exercise recovery

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Aguiar, P. F., Magalhães, S. M., Fonseca, I. A. T., da Costa Santos, V. B., de Matos, M. A., Peixoto, M. F. D., ... Amorim, F. T. (2016). Post-exercise cold water immersion does not alter high intensity interval training-induced exercise performance and Hsp72 responses, but enhances mitochondrial markers. Cell Stress and Chaperones, 21(5), 793-804. https://doi.org/10.1007/s12192-016-0704-6

Post-exercise cold water immersion does not alter high intensity interval training-induced exercise performance and Hsp72 responses, but enhances mitochondrial markers. / Aguiar, Paula Fernandes; Magalhães, Sílvia Mourão; Fonseca, Ivana Alice Teixeira; da Costa Santos, Vanessa Batista; de Matos, Mariana Aguiar; Peixoto, Marco Fabrício Dias; Nakamura, Fábio Yuzo; Crandall, Craig; Araújo, Hygor Nunes; Silveira, Leonardo Reis; Rocha-Vieira, Etel; de Castro Magalhães, Flávio; Amorim, Fabiano Trigueiro.

In: Cell Stress and Chaperones, Vol. 21, No. 5, 01.09.2016, p. 793-804.

Research output: Contribution to journalArticle

Aguiar, PF, Magalhães, SM, Fonseca, IAT, da Costa Santos, VB, de Matos, MA, Peixoto, MFD, Nakamura, FY, Crandall, C, Araújo, HN, Silveira, LR, Rocha-Vieira, E, de Castro Magalhães, F & Amorim, FT 2016, 'Post-exercise cold water immersion does not alter high intensity interval training-induced exercise performance and Hsp72 responses, but enhances mitochondrial markers', Cell Stress and Chaperones, vol. 21, no. 5, pp. 793-804. https://doi.org/10.1007/s12192-016-0704-6
Aguiar, Paula Fernandes ; Magalhães, Sílvia Mourão ; Fonseca, Ivana Alice Teixeira ; da Costa Santos, Vanessa Batista ; de Matos, Mariana Aguiar ; Peixoto, Marco Fabrício Dias ; Nakamura, Fábio Yuzo ; Crandall, Craig ; Araújo, Hygor Nunes ; Silveira, Leonardo Reis ; Rocha-Vieira, Etel ; de Castro Magalhães, Flávio ; Amorim, Fabiano Trigueiro. / Post-exercise cold water immersion does not alter high intensity interval training-induced exercise performance and Hsp72 responses, but enhances mitochondrial markers. In: Cell Stress and Chaperones. 2016 ; Vol. 21, No. 5. pp. 793-804.
@article{d6ce74edfde74f2ea785d1862da2a579,
title = "Post-exercise cold water immersion does not alter high intensity interval training-induced exercise performance and Hsp72 responses, but enhances mitochondrial markers",
abstract = "This study aims to evaluate the effect of regular post-exercise cold water immersion (CWI) on intramuscular markers of cellular stress response and signaling molecules related to mitochondria biogenesis and exercise performance after 4 weeks of high intensity interval training (HIIT). Seventeen healthy subjects were allocated into two groups: control (CON, n = 9) or CWI (n = 8). Each HIIT session consisted of 8–12 cycling exercise stimuli (90–110 {\%} of peak power) for 60 s followed by 75 s of active recovery three times per week, for 4 weeks (12 HIIT sessions). After each HIIT session, the CWI had their lower limbs immersed in cold water (10 °C) for 15 min and the CON recovered at room temperature. Exercise performance was evaluated before and after HIIT by a 15-km cycling time trial. Vastus lateralis biopsies were obtained pre and 72 h post training. Samples were analyzed for heat shock protein 72 kDa (Hsp72), adenosine monophosphate-activated protein kinase (AMPK), and phosphorylated p38 mitogen-activated protein kinase (p-p38 MAPK) assessed by western blot. In addition, the mRNA expression of heat shock factor-1 (HSF-1), peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α), nuclear respiratory factor 1 and 2 (NRF1 and 2), mitochondrial transcription factor A (Tfam), calcium calmodulin-dependent protein kinase 2 (CaMK2) and enzymes citrate synthase (CS), carnitine palmitoyltransferase I (CPT1), and pyruvate dehydrogenase kinase (PDK4) were assessed by real-time PCR. Time to complete the 15-km cycling time trial was reduced with training (p < 0.001), but was not different between groups (p = 0.33). The Hsp72 (p = 0.01), p38 MAPK, and AMPK (p = 0.04) contents increased with training, but were not different between groups (p > 0.05). No differences were observed with training or condition for mRNA expression of PGC-1α (p = 0.31), CPT1 (p = 0.14), CS (p = 0.44), and NRF-2 (p = 0.82). However, HFS-1 (p = 0.007), PDK4 (p = 0.03), and Tfam (p = 0.03) mRNA were higher in CWI. NRF-1 decrease in both groups after training (p = 0.006). CaMK2 decreased with HIIT (p = 0.003) but it was not affected by CWI (p = 0.99). Cold water immersion does not alter HIIT-induced Hsp72, AMPK, p38 MAPK, and exercise performance but was able to increase some markers of cellular stress response and signaling molecules related to mitochondria biogenesis.",
keywords = "Cold water immersion, Heat shock protein, High intensity interval training, Mitochondria biogenesis, Post-exercise recovery",
author = "Aguiar, {Paula Fernandes} and Magalh{\~a}es, {S{\'i}lvia Mour{\~a}o} and Fonseca, {Ivana Alice Teixeira} and {da Costa Santos}, {Vanessa Batista} and {de Matos}, {Mariana Aguiar} and Peixoto, {Marco Fabr{\'i}cio Dias} and Nakamura, {F{\'a}bio Yuzo} and Craig Crandall and Ara{\'u}jo, {Hygor Nunes} and Silveira, {Leonardo Reis} and Etel Rocha-Vieira and {de Castro Magalh{\~a}es}, Fl{\'a}vio and Amorim, {Fabiano Trigueiro}",
year = "2016",
month = "9",
day = "1",
doi = "10.1007/s12192-016-0704-6",
language = "English (US)",
volume = "21",
pages = "793--804",
journal = "Cell Stress and Chaperones",
issn = "1355-8145",
publisher = "Springer Netherlands",
number = "5",

}

TY - JOUR

T1 - Post-exercise cold water immersion does not alter high intensity interval training-induced exercise performance and Hsp72 responses, but enhances mitochondrial markers

AU - Aguiar, Paula Fernandes

AU - Magalhães, Sílvia Mourão

AU - Fonseca, Ivana Alice Teixeira

AU - da Costa Santos, Vanessa Batista

AU - de Matos, Mariana Aguiar

AU - Peixoto, Marco Fabrício Dias

AU - Nakamura, Fábio Yuzo

AU - Crandall, Craig

AU - Araújo, Hygor Nunes

AU - Silveira, Leonardo Reis

AU - Rocha-Vieira, Etel

AU - de Castro Magalhães, Flávio

AU - Amorim, Fabiano Trigueiro

PY - 2016/9/1

Y1 - 2016/9/1

N2 - This study aims to evaluate the effect of regular post-exercise cold water immersion (CWI) on intramuscular markers of cellular stress response and signaling molecules related to mitochondria biogenesis and exercise performance after 4 weeks of high intensity interval training (HIIT). Seventeen healthy subjects were allocated into two groups: control (CON, n = 9) or CWI (n = 8). Each HIIT session consisted of 8–12 cycling exercise stimuli (90–110 % of peak power) for 60 s followed by 75 s of active recovery three times per week, for 4 weeks (12 HIIT sessions). After each HIIT session, the CWI had their lower limbs immersed in cold water (10 °C) for 15 min and the CON recovered at room temperature. Exercise performance was evaluated before and after HIIT by a 15-km cycling time trial. Vastus lateralis biopsies were obtained pre and 72 h post training. Samples were analyzed for heat shock protein 72 kDa (Hsp72), adenosine monophosphate-activated protein kinase (AMPK), and phosphorylated p38 mitogen-activated protein kinase (p-p38 MAPK) assessed by western blot. In addition, the mRNA expression of heat shock factor-1 (HSF-1), peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α), nuclear respiratory factor 1 and 2 (NRF1 and 2), mitochondrial transcription factor A (Tfam), calcium calmodulin-dependent protein kinase 2 (CaMK2) and enzymes citrate synthase (CS), carnitine palmitoyltransferase I (CPT1), and pyruvate dehydrogenase kinase (PDK4) were assessed by real-time PCR. Time to complete the 15-km cycling time trial was reduced with training (p < 0.001), but was not different between groups (p = 0.33). The Hsp72 (p = 0.01), p38 MAPK, and AMPK (p = 0.04) contents increased with training, but were not different between groups (p > 0.05). No differences were observed with training or condition for mRNA expression of PGC-1α (p = 0.31), CPT1 (p = 0.14), CS (p = 0.44), and NRF-2 (p = 0.82). However, HFS-1 (p = 0.007), PDK4 (p = 0.03), and Tfam (p = 0.03) mRNA were higher in CWI. NRF-1 decrease in both groups after training (p = 0.006). CaMK2 decreased with HIIT (p = 0.003) but it was not affected by CWI (p = 0.99). Cold water immersion does not alter HIIT-induced Hsp72, AMPK, p38 MAPK, and exercise performance but was able to increase some markers of cellular stress response and signaling molecules related to mitochondria biogenesis.

AB - This study aims to evaluate the effect of regular post-exercise cold water immersion (CWI) on intramuscular markers of cellular stress response and signaling molecules related to mitochondria biogenesis and exercise performance after 4 weeks of high intensity interval training (HIIT). Seventeen healthy subjects were allocated into two groups: control (CON, n = 9) or CWI (n = 8). Each HIIT session consisted of 8–12 cycling exercise stimuli (90–110 % of peak power) for 60 s followed by 75 s of active recovery three times per week, for 4 weeks (12 HIIT sessions). After each HIIT session, the CWI had their lower limbs immersed in cold water (10 °C) for 15 min and the CON recovered at room temperature. Exercise performance was evaluated before and after HIIT by a 15-km cycling time trial. Vastus lateralis biopsies were obtained pre and 72 h post training. Samples were analyzed for heat shock protein 72 kDa (Hsp72), adenosine monophosphate-activated protein kinase (AMPK), and phosphorylated p38 mitogen-activated protein kinase (p-p38 MAPK) assessed by western blot. In addition, the mRNA expression of heat shock factor-1 (HSF-1), peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α), nuclear respiratory factor 1 and 2 (NRF1 and 2), mitochondrial transcription factor A (Tfam), calcium calmodulin-dependent protein kinase 2 (CaMK2) and enzymes citrate synthase (CS), carnitine palmitoyltransferase I (CPT1), and pyruvate dehydrogenase kinase (PDK4) were assessed by real-time PCR. Time to complete the 15-km cycling time trial was reduced with training (p < 0.001), but was not different between groups (p = 0.33). The Hsp72 (p = 0.01), p38 MAPK, and AMPK (p = 0.04) contents increased with training, but were not different between groups (p > 0.05). No differences were observed with training or condition for mRNA expression of PGC-1α (p = 0.31), CPT1 (p = 0.14), CS (p = 0.44), and NRF-2 (p = 0.82). However, HFS-1 (p = 0.007), PDK4 (p = 0.03), and Tfam (p = 0.03) mRNA were higher in CWI. NRF-1 decrease in both groups after training (p = 0.006). CaMK2 decreased with HIIT (p = 0.003) but it was not affected by CWI (p = 0.99). Cold water immersion does not alter HIIT-induced Hsp72, AMPK, p38 MAPK, and exercise performance but was able to increase some markers of cellular stress response and signaling molecules related to mitochondria biogenesis.

KW - Cold water immersion

KW - Heat shock protein

KW - High intensity interval training

KW - Mitochondria biogenesis

KW - Post-exercise recovery

UR - http://www.scopus.com/inward/record.url?scp=84976337097&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84976337097&partnerID=8YFLogxK

U2 - 10.1007/s12192-016-0704-6

DO - 10.1007/s12192-016-0704-6

M3 - Article

C2 - 27278803

AN - SCOPUS:84976337097

VL - 21

SP - 793

EP - 804

JO - Cell Stress and Chaperones

JF - Cell Stress and Chaperones

SN - 1355-8145

IS - 5

ER -