TY - JOUR
T1 - Postnatal development and the differential expression of presynaptic terminal-associated proteins in the developing retina of the Brazilian opossum, Monodelphis domestica
AU - Greenlee, M. H West
AU - Swanson, J. J.
AU - Simon, J. J.
AU - Elmquist, J. K.
AU - Jacobson, C. D.
AU - Sakaguchi, D. S.
N1 - Funding Information:
The authors wish to thank Dr. R. Jahn of the Howard Hughes Medical Institute, Yale University School of Medicine for his generous gifts of anti-synaptotagmin and Rab3A antibodies; Dr. A. Czemik of the Laboratory of Cellular and Molecular Neuroscience, The Rockefeller University, for his generous gift of anti-synaptophysin (p38) antibody; and Dr. M.C. Wilson of the Department of Pharmacology, Scripps Research Institute for his generous gift of anti-SNAP-25 antibody. The authors also wish to thank C. Hix, T. Folsom, and J. Leonard for critical reading of the manuscript, and J. Todd for expert editorial assistance. This work was partially supported by grants from the National Science Foundation (C.D.J. and D.S.S.), the Whitehall Foundation (C.D.J.), the Iowa State University Biotechnology Council and the Carver Trust (D.S.S.). This manuscript, designated by Iowa State University as J-16753 of the Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa, project number 3205, was supported by Hatch Act and State of Iowa Funds.
PY - 1996/10/23
Y1 - 1996/10/23
N2 - In the present study we have characterized the postnatal (PN) development of the retina in the Brazilian opossum, Monodelphis domestica. Monodelphis, a small, pouchless marsupial, undergoes a protracted period of postnatal development. Using bromodeoxyuridine immunohistochemistry, we have investigated postnatal neurogenesis of the retina. In addition, we have examined the differentiation of the retina by using antibodies directed against the presynaptic terminal-associated proteins synaptotagmin, Rab3A, synaptophysin and synaptosomal-associated protein-25 (SNAP-25), and have characterized their spatial and temporal distribution during postnatal development. This study is the first systematic comparison of the developmental expression of multiple presynaptic terminal-associated proteins in relation to retinal neurogenesis and differentiation. At birth (1PN), the Monodelphis retina was relatively undifferentiated morphologically and birthdating analysis revealed mitotically active cells throughout the retina. The 8PN retina was organized into two cellular layers: an outer region of mitotically active neuroepithelial cells and an inner region of postmitotic cells. The inner plexiform layer formed between 5PN and 10PN, and exhibited unique patterns of immunoreactivity with the antibodies used in this analysis. By 25PN the retina was well laminated, and synaptotagmin-, Rab3A-, synaptophysin- and SNAP-25-like immunoreactivities exhibited distinct and specific patterns within the plexiform layers, although they had not yet achieved their mature, adult patterns. These results indicate that each of these proteins exhibits developmentally regulated changes in its cellular localization, and therefore may play important roles during morphogenesis and synaptogenesis of the vertebrate retina.
AB - In the present study we have characterized the postnatal (PN) development of the retina in the Brazilian opossum, Monodelphis domestica. Monodelphis, a small, pouchless marsupial, undergoes a protracted period of postnatal development. Using bromodeoxyuridine immunohistochemistry, we have investigated postnatal neurogenesis of the retina. In addition, we have examined the differentiation of the retina by using antibodies directed against the presynaptic terminal-associated proteins synaptotagmin, Rab3A, synaptophysin and synaptosomal-associated protein-25 (SNAP-25), and have characterized their spatial and temporal distribution during postnatal development. This study is the first systematic comparison of the developmental expression of multiple presynaptic terminal-associated proteins in relation to retinal neurogenesis and differentiation. At birth (1PN), the Monodelphis retina was relatively undifferentiated morphologically and birthdating analysis revealed mitotically active cells throughout the retina. The 8PN retina was organized into two cellular layers: an outer region of mitotically active neuroepithelial cells and an inner region of postmitotic cells. The inner plexiform layer formed between 5PN and 10PN, and exhibited unique patterns of immunoreactivity with the antibodies used in this analysis. By 25PN the retina was well laminated, and synaptotagmin-, Rab3A-, synaptophysin- and SNAP-25-like immunoreactivities exhibited distinct and specific patterns within the plexiform layers, although they had not yet achieved their mature, adult patterns. These results indicate that each of these proteins exhibits developmentally regulated changes in its cellular localization, and therefore may play important roles during morphogenesis and synaptogenesis of the vertebrate retina.
KW - Rab3A
KW - bromodeoxyuridine
KW - synaptophysin (p38)
KW - synaptosomal-associated protein-25 (SNAP-25)
KW - synaptotagmin (p65)
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U2 - 10.1016/S0165-3806(96)00102-2
DO - 10.1016/S0165-3806(96)00102-2
M3 - Article
C2 - 8922678
AN - SCOPUS:0030599406
SN - 0165-3806
VL - 96
SP - 159
EP - 172
JO - Developmental Brain Research
JF - Developmental Brain Research
IS - 1-2
ER -