Postnatal estradiol up-regulates lung nitric oxide synthases and improves lung function in bronchopulmonary dysplasia

Donald C. McCurnin, Richard A. Pierce, Brigham C. Willis, Ling Yi Chang, Bradley A. Yoder, Ivan S. Yuhanna, Philip L. Ballard, Ronald I. Clyman, Nahid Waleh, William Maniscalco, James D. Crapo, Peter H. Grubb, Philip W. Shaul

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Rationale. Nitric oxide (NO) plays an important role in lung development and perinatal lung function, and pulmonary NO synthases (NOS) are decreased in bronchopulmonary dysplasia (BPD) following preterm birth. Fetal estradiol levels increase during late gestation and estradiol up-regulates NOS, suggesting that after preterm birth estradiol deprivation causes attenuated lung NOS resulting in impaired pulmonary function. Objective. To test the effects of postnatal estradiol administration in a primate model of BPD over 14 days after delivery at 125 days of gestation (term = 185 d). Methods. Cardiopulmonary function was assessed by echocardiog- raphy and whole body plethysmography. Lung morphometric and histopathologic analyses were performed, and NOS enzymatic activity and abundance were measured. Measurements and Main Results. Estradiol caused an increase in blood pressure and ductus arteriosus closure. Expiratory resistance and lung compliance were also improved, and this occurred before spontaneous ductal closure. Furthermore, both oxygenation and ventilation indices were improved with estradiol, and the changes in lung function and ventilatory support requirements persisted throughout the study period. Whereas estradiol had negligible effect on indicators of lung inflammation and on lung structure assessed after the initial 14 days of ventilatory support, it caused an increase in lung neuronal and endothelial NOS enzymatic activity. Conclusions. In a primate model of BPD, postnatal estradiol treatment had favorable cardiovascular impact, enhanced pulmonary function, and lowered requirements for ventilatory support in association with an up-regulation of lung NOS. Estradiol may bean efficacious postnatal therapy to improve lung function and outcome in preterm infants.

Original languageEnglish (US)
Pages (from-to)492-500
Number of pages9
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume179
Issue number6
DOIs
StatePublished - Mar 15 2009

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Bronchopulmonary Dysplasia
Nitric Oxide Synthase
Estradiol
Up-Regulation
Lung
Premature Birth
Primates
Whole Body Plethysmography
Lung Compliance
Ductus Arteriosus
Pregnancy
Premature Infants
Ventilation
Echocardiography
Pneumonia
Nitric Oxide

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

Cite this

Postnatal estradiol up-regulates lung nitric oxide synthases and improves lung function in bronchopulmonary dysplasia. / McCurnin, Donald C.; Pierce, Richard A.; Willis, Brigham C.; Chang, Ling Yi; Yoder, Bradley A.; Yuhanna, Ivan S.; Ballard, Philip L.; Clyman, Ronald I.; Waleh, Nahid; Maniscalco, William; Crapo, James D.; Grubb, Peter H.; Shaul, Philip W.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 179, No. 6, 15.03.2009, p. 492-500.

Research output: Contribution to journalArticle

McCurnin, DC, Pierce, RA, Willis, BC, Chang, LY, Yoder, BA, Yuhanna, IS, Ballard, PL, Clyman, RI, Waleh, N, Maniscalco, W, Crapo, JD, Grubb, PH & Shaul, PW 2009, 'Postnatal estradiol up-regulates lung nitric oxide synthases and improves lung function in bronchopulmonary dysplasia', American Journal of Respiratory and Critical Care Medicine, vol. 179, no. 6, pp. 492-500. https://doi.org/10.1164/rccm.200805-794OC
McCurnin, Donald C. ; Pierce, Richard A. ; Willis, Brigham C. ; Chang, Ling Yi ; Yoder, Bradley A. ; Yuhanna, Ivan S. ; Ballard, Philip L. ; Clyman, Ronald I. ; Waleh, Nahid ; Maniscalco, William ; Crapo, James D. ; Grubb, Peter H. ; Shaul, Philip W. / Postnatal estradiol up-regulates lung nitric oxide synthases and improves lung function in bronchopulmonary dysplasia. In: American Journal of Respiratory and Critical Care Medicine. 2009 ; Vol. 179, No. 6. pp. 492-500.
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abstract = "Rationale. Nitric oxide (NO) plays an important role in lung development and perinatal lung function, and pulmonary NO synthases (NOS) are decreased in bronchopulmonary dysplasia (BPD) following preterm birth. Fetal estradiol levels increase during late gestation and estradiol up-regulates NOS, suggesting that after preterm birth estradiol deprivation causes attenuated lung NOS resulting in impaired pulmonary function. Objective. To test the effects of postnatal estradiol administration in a primate model of BPD over 14 days after delivery at 125 days of gestation (term = 185 d). Methods. Cardiopulmonary function was assessed by echocardiog- raphy and whole body plethysmography. Lung morphometric and histopathologic analyses were performed, and NOS enzymatic activity and abundance were measured. Measurements and Main Results. Estradiol caused an increase in blood pressure and ductus arteriosus closure. Expiratory resistance and lung compliance were also improved, and this occurred before spontaneous ductal closure. Furthermore, both oxygenation and ventilation indices were improved with estradiol, and the changes in lung function and ventilatory support requirements persisted throughout the study period. Whereas estradiol had negligible effect on indicators of lung inflammation and on lung structure assessed after the initial 14 days of ventilatory support, it caused an increase in lung neuronal and endothelial NOS enzymatic activity. Conclusions. In a primate model of BPD, postnatal estradiol treatment had favorable cardiovascular impact, enhanced pulmonary function, and lowered requirements for ventilatory support in association with an up-regulation of lung NOS. Estradiol may bean efficacious postnatal therapy to improve lung function and outcome in preterm infants.",
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AU - McCurnin, Donald C.

AU - Pierce, Richard A.

AU - Willis, Brigham C.

AU - Chang, Ling Yi

AU - Yoder, Bradley A.

AU - Yuhanna, Ivan S.

AU - Ballard, Philip L.

AU - Clyman, Ronald I.

AU - Waleh, Nahid

AU - Maniscalco, William

AU - Crapo, James D.

AU - Grubb, Peter H.

AU - Shaul, Philip W.

PY - 2009/3/15

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N2 - Rationale. Nitric oxide (NO) plays an important role in lung development and perinatal lung function, and pulmonary NO synthases (NOS) are decreased in bronchopulmonary dysplasia (BPD) following preterm birth. Fetal estradiol levels increase during late gestation and estradiol up-regulates NOS, suggesting that after preterm birth estradiol deprivation causes attenuated lung NOS resulting in impaired pulmonary function. Objective. To test the effects of postnatal estradiol administration in a primate model of BPD over 14 days after delivery at 125 days of gestation (term = 185 d). Methods. Cardiopulmonary function was assessed by echocardiog- raphy and whole body plethysmography. Lung morphometric and histopathologic analyses were performed, and NOS enzymatic activity and abundance were measured. Measurements and Main Results. Estradiol caused an increase in blood pressure and ductus arteriosus closure. Expiratory resistance and lung compliance were also improved, and this occurred before spontaneous ductal closure. Furthermore, both oxygenation and ventilation indices were improved with estradiol, and the changes in lung function and ventilatory support requirements persisted throughout the study period. Whereas estradiol had negligible effect on indicators of lung inflammation and on lung structure assessed after the initial 14 days of ventilatory support, it caused an increase in lung neuronal and endothelial NOS enzymatic activity. Conclusions. In a primate model of BPD, postnatal estradiol treatment had favorable cardiovascular impact, enhanced pulmonary function, and lowered requirements for ventilatory support in association with an up-regulation of lung NOS. Estradiol may bean efficacious postnatal therapy to improve lung function and outcome in preterm infants.

AB - Rationale. Nitric oxide (NO) plays an important role in lung development and perinatal lung function, and pulmonary NO synthases (NOS) are decreased in bronchopulmonary dysplasia (BPD) following preterm birth. Fetal estradiol levels increase during late gestation and estradiol up-regulates NOS, suggesting that after preterm birth estradiol deprivation causes attenuated lung NOS resulting in impaired pulmonary function. Objective. To test the effects of postnatal estradiol administration in a primate model of BPD over 14 days after delivery at 125 days of gestation (term = 185 d). Methods. Cardiopulmonary function was assessed by echocardiog- raphy and whole body plethysmography. Lung morphometric and histopathologic analyses were performed, and NOS enzymatic activity and abundance were measured. Measurements and Main Results. Estradiol caused an increase in blood pressure and ductus arteriosus closure. Expiratory resistance and lung compliance were also improved, and this occurred before spontaneous ductal closure. Furthermore, both oxygenation and ventilation indices were improved with estradiol, and the changes in lung function and ventilatory support requirements persisted throughout the study period. Whereas estradiol had negligible effect on indicators of lung inflammation and on lung structure assessed after the initial 14 days of ventilatory support, it caused an increase in lung neuronal and endothelial NOS enzymatic activity. Conclusions. In a primate model of BPD, postnatal estradiol treatment had favorable cardiovascular impact, enhanced pulmonary function, and lowered requirements for ventilatory support in association with an up-regulation of lung NOS. Estradiol may bean efficacious postnatal therapy to improve lung function and outcome in preterm infants.

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