Postnatal xenogeneic B-cell tolerance in swine following in utero intraportal antigen exposure

Nalu Navarro Alvarez, Alexander Zhu, Ronald S. Arellano, Mark A. Randolph, Michael Duggan, John Scott Arn, Christene A. Huang, David H. Sachs, Parsia A. Vagefi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background The objective of this study was to investigate the humoral immune response to xenogeneic antigens administered during the fetal state utilizing a baboon-to-pig model. Methods Nine fetuses from an alpha-1,3-galactosyltransferase gene knockout (GalT-KO) MGH-miniature swine sow underwent transuterine ultrasound-guided intraportal injection of T-cell depleted baboon bone marrow (B-BM) at mid-gestation. Two juvenile GalT-KO swine undergoing direct B-BM intraportal injection were used as controls. Results Postnatal humoral tolerance was induced in the long-term surviving piglets as demonstrated by the absence of any antibody response to baboon donor cells. In addition, a second intraportal B-BM administration at 2.5 months post-birth led to no antibody formation despite re-exposure to xenogeneic antigens. This B-cell unresponsiveness was abrogated only when the animal was exposed subcutaneously to third-party xenogeneic and allogeneic antigens, suggesting that the previously achieved humoral non-responsiveness was donor specific. In comparison, the two juvenile GalT-KO control swine demonstrated increasing anti-baboon IgM and IgG levels following intraportal injection. Conclusions In summary, xenogeneic B-cell tolerance was induced through in utero intraportal exposure to donor cells and this tolerance persisted following postnatal rechallenge with donor B-BM, but was lost on exposure to third-party antigen, possibly as a result of cross-reactive antibody formation.

Original languageEnglish (US)
Pages (from-to)368-378
Number of pages11
JournalXenotransplantation
Volume22
Issue number5
DOIs
StatePublished - Sep 1 2015
Externally publishedYes

Fingerprint

Papio
B-Lymphocytes
Swine
Antigens
Heterophile Antigens
Gene Knockout Techniques
Bone Marrow
Antibody Formation
Galactosyltransferases
Injections
Miniature Swine
Humoral Immunity
Fetus
Parturition
T-Lymphocytes
Pregnancy

Keywords

  • B-cell tolerance
  • in utero tolerance
  • xenotransplant

ASJC Scopus subject areas

  • Immunology
  • Transplantation

Cite this

Navarro Alvarez, N., Zhu, A., Arellano, R. S., Randolph, M. A., Duggan, M., Scott Arn, J., ... Vagefi, P. A. (2015). Postnatal xenogeneic B-cell tolerance in swine following in utero intraportal antigen exposure. Xenotransplantation, 22(5), 368-378. https://doi.org/10.1111/xen.12186

Postnatal xenogeneic B-cell tolerance in swine following in utero intraportal antigen exposure. / Navarro Alvarez, Nalu; Zhu, Alexander; Arellano, Ronald S.; Randolph, Mark A.; Duggan, Michael; Scott Arn, John; Huang, Christene A.; Sachs, David H.; Vagefi, Parsia A.

In: Xenotransplantation, Vol. 22, No. 5, 01.09.2015, p. 368-378.

Research output: Contribution to journalArticle

Navarro Alvarez, N, Zhu, A, Arellano, RS, Randolph, MA, Duggan, M, Scott Arn, J, Huang, CA, Sachs, DH & Vagefi, PA 2015, 'Postnatal xenogeneic B-cell tolerance in swine following in utero intraportal antigen exposure', Xenotransplantation, vol. 22, no. 5, pp. 368-378. https://doi.org/10.1111/xen.12186
Navarro Alvarez N, Zhu A, Arellano RS, Randolph MA, Duggan M, Scott Arn J et al. Postnatal xenogeneic B-cell tolerance in swine following in utero intraportal antigen exposure. Xenotransplantation. 2015 Sep 1;22(5):368-378. https://doi.org/10.1111/xen.12186
Navarro Alvarez, Nalu ; Zhu, Alexander ; Arellano, Ronald S. ; Randolph, Mark A. ; Duggan, Michael ; Scott Arn, John ; Huang, Christene A. ; Sachs, David H. ; Vagefi, Parsia A. / Postnatal xenogeneic B-cell tolerance in swine following in utero intraportal antigen exposure. In: Xenotransplantation. 2015 ; Vol. 22, No. 5. pp. 368-378.
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