Postrecruitment regulation of RNA polymerase II directs rapid signaling responses at the promoters of estrogen target genes

Miltiadis Kininis, Gary D. Isaacs, Leighton J. Core, Nasun Hah, W. Lee Kraus

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Under classical models for signal-dependent transcription in eukaryotes, DNA-binding activator proteins regulate the recruitment of RNA polymerase II (Pol II) to a set of target promoters. However, recent studies, as well as our results herein, show that Pol II is widely distributed (i.e., "preloaded") at the promoters of many genes prior to specific signaling events. How Pol II recruitment and Pol II preloading fit within a unified model of gene regulation is unclear. In addition, the mechanisms through which cellular signals activate preloaded Pol II across mammalian genomes remain largely unknown. We show here that the predominant genomic outcome of estrogen signaling is the postrecruitment regulation of Pol II activity at target gene promoters, likely through specific changes in Pol II phosphorylation rather than through recruitment of Pol II to the promoters. Furthermore, we show that negative elongation factor binds to estrogen target promoters in conjunction with preloaded Pol II and represses gene expression until the appropriate signal is received. Finally, our studies reveal that the estrogen-dependent activation of preloaded Pol II facilitates rapid gene regulatory responses which play important physiological roles in regulating estrogen signaling itself. Our results reveal a broad use of postrecruitment Pol II regulation by the estrogen signaling pathway, a mode of regulation that is likely to apply to a wide variety of signal-regulated pathways.

Original languageEnglish (US)
Pages (from-to)1123-1133
Number of pages11
JournalMolecular and Cellular Biology
Volume29
Issue number5
DOIs
StatePublished - Mar 2009

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RNA Polymerase II
Estrogens
Genes
DNA-Binding Proteins
Regulator Genes
Eukaryota
Signal Transduction
Phosphorylation
Genome
Gene Expression

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Postrecruitment regulation of RNA polymerase II directs rapid signaling responses at the promoters of estrogen target genes. / Kininis, Miltiadis; Isaacs, Gary D.; Core, Leighton J.; Hah, Nasun; Kraus, W. Lee.

In: Molecular and Cellular Biology, Vol. 29, No. 5, 03.2009, p. 1123-1133.

Research output: Contribution to journalArticle

Kininis, Miltiadis ; Isaacs, Gary D. ; Core, Leighton J. ; Hah, Nasun ; Kraus, W. Lee. / Postrecruitment regulation of RNA polymerase II directs rapid signaling responses at the promoters of estrogen target genes. In: Molecular and Cellular Biology. 2009 ; Vol. 29, No. 5. pp. 1123-1133.
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