Posttraumatic Chondrocyte Apoptosis in the Murine Xiphoid

Christopher G. Davis, Eric Eisner, Margaret McGlynn, John M. Shelton, James Richardson, Joseph Borrelli, Christopher C T Chen

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Objective. To demonstrate posttraumatic chondrocyte apoptosis in the murine xiphoid after a crush-type injury and to ultimately determine the pathway (i.e., intrinsic or extrinsic) by which chondrocytes undergo apoptosis in response to mechanical injury. Design. The xiphoids of adult female wild-type mice were injured with the use of a modified Kelly clamp. Postinjury xiphoid cartilage was analyzed via 3 well-described independent means of assessing apoptosis in chondrocytes: hematoxylin and eosin staining, terminal deoxynucleotidyl transferase dUTP nick end labeling assay, and activated caspase-3 staining. Results. Injured specimens contained many chondrocytes with evidence of apoptosis, which is characterized by cell shrinkage, chromatin condensation, nuclear fragmentation, and the liberation of apoptotic bodies. There was a statistically significant increase in the number of chondrocytes undergoing apoptosis in the injured specimens as compared with the uninjured specimens. Conclusions. Chondrocytes can be stimulated to undergo apoptosis as a result of mechanical injury. These experiments involving predominantly cartilaginous murine xiphoid in vivo establish a baseline for future investigations that employ the genetic and therapeutic modulation of chondrocyte apoptosis in response to mechanical injury.

Original languageEnglish (US)
Pages (from-to)345-353
Number of pages9
JournalCartilage
Volume4
Issue number4
DOIs
StatePublished - Oct 2013

Keywords

  • apoptosis
  • chondrocytes
  • injury
  • murine
  • xiphoid

ASJC Scopus subject areas

  • Immunology and Allergy
  • Biomedical Engineering
  • Physical Therapy, Sports Therapy and Rehabilitation

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