Abstract
Effective drugs are urgently needed for the treatment of advanced neuroblastoma refractory to conventional chemotherapy. Pyrrolidine dithiocarbamate (PDTC) is a copper-binding ligand, which showed cytotoxicity on many human tumor cells after binding with copper ions. In this study, we synthesized a copper-PDTC complex, which was characterized as a Cu(PDTC)2 complex, with elemental analyses (Fourier transform infrared, electrospray ionization mass spectra, and ultraviolet-visible spectroscopy). The Cu(PDTC)2 complex suppressed the proliferation of BE(2)C cells, a human neuroblastoma cell line, with an IC50 of 8.0 μmol/l, which was more potent than cisplatin (IC50 of 80 μmol/l). Treatment of BE(2)C cells with the Cu(PDTC)2 complex caused the S-phase arrest of cell cycle progression, cellular apoptosis, and necrosis, and increased the expression of p53 protein. The Cu(PDTC)2 complex holds potential as a new drug for the treatment of refractory neuroblastoma in children.
Original language | English (US) |
---|---|
Pages (from-to) | 125-132 |
Number of pages | 8 |
Journal | Anti-Cancer Drugs |
Volume | 19 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2008 |
Keywords
- Apoptosis
- Cell cycle
- Copper
- Neuroblastoma
- Pyrrolidine dithiocarbamate
ASJC Scopus subject areas
- Oncology
- Pharmacology
- Pharmacology (medical)
- Cancer Research