Precisely ordered phosphorylation reactions in the p38 mitogen-activated protein (MAP) kinase cascade

John M. Humphreys, Alexander T. Piala, Radha Akella, Haixia He, Elizabeth J. Goldsmith

Research output: Contribution to journalArticle

18 Scopus citations


Background: MAPK cascades are signaling modules that function as switch generators. Results: In vitro phosphorylation in the p38 MAPK cascade tracked by LC-MS/MS revealed specific phosphorylation intermediates at each level. Conclusion: The p38 MAPK cascade reactions occur through intermediates MEK6/ST* and p38α/TY*. Significance: The precise order of reactions may contribute to the diverse kinetic outputs of the cascades, including those with large Hill coefficients.

Original languageEnglish (US)
Pages (from-to)23322-23330
Number of pages9
JournalJournal of Biological Chemistry
Issue number32
StatePublished - Aug 9 2013


ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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