Predicting potential miRNA target sites within gene promoters

Scott T. Younger, Alexander Pertsemlidis, David R. Corey

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

Synthetic small duplex RNAs that are complementary to gene promoters can activate or inhibit target gene expression. The potency and robustness of gene modulation by these RNAs suggests that natural mechanisms may exist to facilitate recognition of sequences within gene promoters by endogenous small RNAs. Here, we describe computational methods for identifying potential miRNA target sites within gene promoters. These methods will facilitate investigations of whether miRNAs interact with sequences outside of 3′-untranslated regions and suggest new targets for the design of synthetic modulators of gene expression.

Original languageEnglish (US)
Pages (from-to)3791-3794
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume19
Issue number14
DOIs
StatePublished - Jul 15 2009

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Keywords

  • Antigene
  • Duplex RNA
  • Gene promoter
  • Hybridization
  • MicroRNA
  • Noncoding RNA
  • RNAi
  • miRNA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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