Abstract
The transcription pattern of the heavy chain immunoglobulin gene locus was analyzed in a 6‐month‐old female with agammaglobulinemia characterized by the absence of mature B cells in peripheral blood, arrested B cell development in the bone marrow and lack of germinal center development. DNA sequencing provided no evidence of mutations within the coding region of the Bruton's tyrosine kinase gene. Polymerase chain reaction‐generated cDNA libraries from blood and bone marrow were screened initially using JH and CH oligodeoxynucleotide probes and VH family‐specific probes. Only 10% of the transcripts constituted mature VDJCμ recombinations. Ninety percent of the cDNA were sterile immunoglobulin transcripts comprised of: DJCμ (DH−JHCμ), JCμ (JH−Cμ), ECμ (enhancer spliced to Cμ), SCμ and ICμ [corresponding to switch (S) and intron (I) regions spliced to Cμ]. In the mature immunoglobulin transcripts, VH use indicated germline expression with little evidence of somatic mutation. All cDNA were of the Cμ type. Different D segments, D‐D joining events and unknown D‐like elements were noted in the DJCμ and VDJCμ transcripts. This pattern of immunoglobulin rearrangements, along with the phenotypic cell surface antigen characteristics (CD19−), suggest that an earlier arrest in B cell development than is characteristic of Bruton's X‐linked agammaglobulinemia has occurred in this patient.
Original language | English (US) |
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Pages (from-to) | 809-815 |
Number of pages | 7 |
Journal | European Journal of Immunology |
Volume | 25 |
Issue number | 3 |
DOIs | |
State | Published - Mar 1995 |
Keywords
- Immunodeficiency
- Immunoglobulin
- Junctional diversity
- V family
- mRNA transcript
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology