Predominance of sterile immunoglobulin transcripts in a female phenotypically resembling Bruton's agammaglobulinemia

Maite De La Morena, Robert N. Haire, Yuko Ohta, Robert P. Nelson, Ronda T. Litman, Noorbibi K. Day, Robert A. Good, Gary W. Litman

Research output: Contribution to journalArticle

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Abstract

The transcription pattern of the heavy chain immunoglobulin gene locus was analyzed in a 6-month-old female with agammaglobulinemia characterized by the absence of mature B cells in peripheral blood, arrested B cell development in the bone marrow and lack of germinal center development. DNA sequencing provided no evidence of mutations within the coding region of the Bruton's tyrosine kinase gene. Polymerase chain reaction-generated cDNA libraries from blood and bone marrow were screened initially using J(H) and C(H) oligodeoxynucleotide probes and V(H) family-specific probes. Only 10% of the transcripts constituted mature VDJCμ recombinations. Ninety percent of the cDNA were sterile immunoglobulin transcripts comprised of: DJCμ (D(H)-J(H)Cμ), JCμ (J(H)-Cμ), ECμ (enhancer spliced to Cμ), SCμ and ICμ [corresponding to switch (S) and intron (I) regions spliced to Cμ] In the mature immunoglobulin transcripts, V(H) use indicated germline expression with little evidence of somatic mutation. All cDNA were of the Cμ type. Different D segments, D-D joining events and unknown D-like elements were noted in the DJCμ and VDJCμ transcripts. This pattern of immunoglobulin rearrangements, along with the phenotypic cell surface antigen characteristics (CD19-), suggest that an earlier arrest in B cell development than is characteristic of Bruton's X-linked agammaglobulinemia has occurred in this patient.

Original languageEnglish (US)
Pages (from-to)809-815
Number of pages7
JournalEuropean Journal of Immunology
Volume25
Issue number3
DOIs
StatePublished - Mar 1995

Fingerprint

Immunoglobulins
B-Lymphocytes
Complementary DNA
Bone Marrow
Immunoglobulin Variable Region
Immunoglobulin Heavy Chain Genes
Agammaglobulinemia
Mutation
Germinal Center
Oligodeoxyribonucleotides
Surface Antigens
Gene Library
DNA Sequence Analysis
Introns
Genetic Recombination
Polymerase Chain Reaction
Genes
Bruton type agammaglobulinemia
Agammaglobulinaemia tyrosine kinase
Fumigant 93

Keywords

  • Immunodeficiency
  • Immunoglobulin
  • Junctional diversity
  • mRNA transcript
  • V(H) family

ASJC Scopus subject areas

  • Immunology

Cite this

De La Morena, M., Haire, R. N., Ohta, Y., Nelson, R. P., Litman, R. T., Day, N. K., ... Litman, G. W. (1995). Predominance of sterile immunoglobulin transcripts in a female phenotypically resembling Bruton's agammaglobulinemia. European Journal of Immunology, 25(3), 809-815. https://doi.org/10.1002/eji.1830250327

Predominance of sterile immunoglobulin transcripts in a female phenotypically resembling Bruton's agammaglobulinemia. / De La Morena, Maite; Haire, Robert N.; Ohta, Yuko; Nelson, Robert P.; Litman, Ronda T.; Day, Noorbibi K.; Good, Robert A.; Litman, Gary W.

In: European Journal of Immunology, Vol. 25, No. 3, 03.1995, p. 809-815.

Research output: Contribution to journalArticle

De La Morena, M, Haire, RN, Ohta, Y, Nelson, RP, Litman, RT, Day, NK, Good, RA & Litman, GW 1995, 'Predominance of sterile immunoglobulin transcripts in a female phenotypically resembling Bruton's agammaglobulinemia', European Journal of Immunology, vol. 25, no. 3, pp. 809-815. https://doi.org/10.1002/eji.1830250327
De La Morena, Maite ; Haire, Robert N. ; Ohta, Yuko ; Nelson, Robert P. ; Litman, Ronda T. ; Day, Noorbibi K. ; Good, Robert A. ; Litman, Gary W. / Predominance of sterile immunoglobulin transcripts in a female phenotypically resembling Bruton's agammaglobulinemia. In: European Journal of Immunology. 1995 ; Vol. 25, No. 3. pp. 809-815.
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AU - Nelson, Robert P.

AU - Litman, Ronda T.

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N2 - The transcription pattern of the heavy chain immunoglobulin gene locus was analyzed in a 6-month-old female with agammaglobulinemia characterized by the absence of mature B cells in peripheral blood, arrested B cell development in the bone marrow and lack of germinal center development. DNA sequencing provided no evidence of mutations within the coding region of the Bruton's tyrosine kinase gene. Polymerase chain reaction-generated cDNA libraries from blood and bone marrow were screened initially using J(H) and C(H) oligodeoxynucleotide probes and V(H) family-specific probes. Only 10% of the transcripts constituted mature VDJCμ recombinations. Ninety percent of the cDNA were sterile immunoglobulin transcripts comprised of: DJCμ (D(H)-J(H)Cμ), JCμ (J(H)-Cμ), ECμ (enhancer spliced to Cμ), SCμ and ICμ [corresponding to switch (S) and intron (I) regions spliced to Cμ] In the mature immunoglobulin transcripts, V(H) use indicated germline expression with little evidence of somatic mutation. All cDNA were of the Cμ type. Different D segments, D-D joining events and unknown D-like elements were noted in the DJCμ and VDJCμ transcripts. This pattern of immunoglobulin rearrangements, along with the phenotypic cell surface antigen characteristics (CD19-), suggest that an earlier arrest in B cell development than is characteristic of Bruton's X-linked agammaglobulinemia has occurred in this patient.

AB - The transcription pattern of the heavy chain immunoglobulin gene locus was analyzed in a 6-month-old female with agammaglobulinemia characterized by the absence of mature B cells in peripheral blood, arrested B cell development in the bone marrow and lack of germinal center development. DNA sequencing provided no evidence of mutations within the coding region of the Bruton's tyrosine kinase gene. Polymerase chain reaction-generated cDNA libraries from blood and bone marrow were screened initially using J(H) and C(H) oligodeoxynucleotide probes and V(H) family-specific probes. Only 10% of the transcripts constituted mature VDJCμ recombinations. Ninety percent of the cDNA were sterile immunoglobulin transcripts comprised of: DJCμ (D(H)-J(H)Cμ), JCμ (J(H)-Cμ), ECμ (enhancer spliced to Cμ), SCμ and ICμ [corresponding to switch (S) and intron (I) regions spliced to Cμ] In the mature immunoglobulin transcripts, V(H) use indicated germline expression with little evidence of somatic mutation. All cDNA were of the Cμ type. Different D segments, D-D joining events and unknown D-like elements were noted in the DJCμ and VDJCμ transcripts. This pattern of immunoglobulin rearrangements, along with the phenotypic cell surface antigen characteristics (CD19-), suggest that an earlier arrest in B cell development than is characteristic of Bruton's X-linked agammaglobulinemia has occurred in this patient.

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