Predominant expression of the mitochondrial dicarboxylate carrier in white adipose tissue

Kallol Das, Renée Y. Lewis, Terry P. Combatsiaris, Ying Lin, Lawrence Shapiro, Maureen J. Charron, Philipp E. Scherer

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We report the identification of a novel mouse protein closely related to the family of mitochondrial uncoupling proteins and the oxoglutarate carrier. The cDNA encodes a protein of 287 amino acids that shares all the hallmark features of the mitochondrial transporter superfamily, including six predicted transmembrane domains. It is nearly identical to the sequence recently reported for the rat mitochondrial dicarboxylate carrier (DIC). We find that murine DIC (mDIC) is expressed at very high levels in mitochondria of white adipocytes and is strongly induced in the course of 3T3-L1 adipogenesis. To determine the consequences of the presence of mDIC on the mitochondrial membrane potential, we transiently expressed mDIC in 293-T cells. Over-expression of mDIC leads to significant mitochondrial hyperpolarization. In addition, exposure to cold down-regulates mDIC levels in vivo. In contrast, free fatty acids lead to an up-regulation of mDIC protein in 3T3-L1 adipocytes. This is the first report demonstrating preferential expression in white adipose tissue of any mitochondrial transporter. However, it remains to be determined which metabolic pathways most critically depend on high level expression of mDIC in the adipocyte.

Original languageEnglish (US)
Pages (from-to)313-320
Number of pages8
JournalBiochemical Journal
Volume344
Issue number2
DOIs
StatePublished - Dec 1 1999

Fingerprint

Dicarboxylic Acid Transporters
White Adipose Tissue
Dacarbazine
Tissue
Adipocytes
Proteins
White Adipocytes
Adipogenesis
Mitochondria
T-cells
Mitochondrial Membrane Potential
Metabolic Networks and Pathways
Nonesterified Fatty Acids
Rats
Up-Regulation
Down-Regulation
Complementary DNA
Membranes
T-Lymphocytes
Amino Acids

Keywords

  • Adipocyte
  • Membrane potential
  • Tissue-specific expression

ASJC Scopus subject areas

  • Biochemistry

Cite this

Predominant expression of the mitochondrial dicarboxylate carrier in white adipose tissue. / Das, Kallol; Lewis, Renée Y.; Combatsiaris, Terry P.; Lin, Ying; Shapiro, Lawrence; Charron, Maureen J.; Scherer, Philipp E.

In: Biochemical Journal, Vol. 344, No. 2, 01.12.1999, p. 313-320.

Research output: Contribution to journalArticle

Das, K, Lewis, RY, Combatsiaris, TP, Lin, Y, Shapiro, L, Charron, MJ & Scherer, PE 1999, 'Predominant expression of the mitochondrial dicarboxylate carrier in white adipose tissue', Biochemical Journal, vol. 344, no. 2, pp. 313-320. https://doi.org/10.1042/0264-6021:3440313
Das K, Lewis RY, Combatsiaris TP, Lin Y, Shapiro L, Charron MJ et al. Predominant expression of the mitochondrial dicarboxylate carrier in white adipose tissue. Biochemical Journal. 1999 Dec 1;344(2):313-320. https://doi.org/10.1042/0264-6021:3440313
Das, Kallol ; Lewis, Renée Y. ; Combatsiaris, Terry P. ; Lin, Ying ; Shapiro, Lawrence ; Charron, Maureen J. ; Scherer, Philipp E. / Predominant expression of the mitochondrial dicarboxylate carrier in white adipose tissue. In: Biochemical Journal. 1999 ; Vol. 344, No. 2. pp. 313-320.
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