Preferential survival of CD8 T and NK cells expressing high levels of CD94

Anasuya Gunturi, Rance E. Berg, James Forman

Research output: Contribution to journalArticle

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Abstract

The Qa-1b/Qdm tetramer binds to CD94/NKG2 receptors expressed at high levels on ∼50% of murine NK cells. Although very few CD8 T cells from naive mice express CD94/NKG2 receptors, ∼50% of CD8 T cells taken from mice undergoing a secondary response against Listeria monocytogenes (LM) are CD94high and bind the tetramer. Although CD94int NK cells do not bind the tetramer, CD94int CD8 T cells do, and this binding is dependent on the CD8 coreceptor. We found that the extent of apoptosis in CD8 T and NK cells was inversely related to the expression of CD94, with lower levels of apoptosis seen in CD94high cells after 1-3 days of culture. The difference in CD8 T cell survival was evident as early as 6 h after culture and persisted until nearly all the CD94neg/int cells were apoptotic by 48 h. In contrast, expression of inhibitory Ly-49A,G2,C/I molecules was associated with higher levels of apoptosis. Cross-linking CD94/NKG2 receptors on CD8 T cells from a mouse undergoing an LM infection further reduced the percentage of apoptotic cells on the CD94-expressing populations, while cross-linking Ly-49I had no effect on CD8 T cells expressing Ly-49I. Cross-linking CD3 on CD8 T cells from a mouse undergoing a secondary LM infection increases the extent of apoptosis, but this is prevented by cross-linking CD94/NKG2 receptors at the same time. Similar results were observed with NK cells in that the CD94high population displayed less apoptosis than CD94int cells after 1-3 days in culture. Therefore, the expression of CD94/NKG2 is correlated with a lower level of apoptosis and may play an important role in the maintenance of CD8 T and NK cells.

Original languageEnglish (US)
Pages (from-to)1737-1745
Number of pages9
JournalJournal of Immunology
Volume170
Issue number4
DOIs
StatePublished - Feb 15 2003

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Natural Killer Cells
T-Lymphocytes
Apoptosis
Listeria monocytogenes
Listeriosis
Population
Cell Survival
Maintenance

ASJC Scopus subject areas

  • Immunology

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Preferential survival of CD8 T and NK cells expressing high levels of CD94. / Gunturi, Anasuya; Berg, Rance E.; Forman, James.

In: Journal of Immunology, Vol. 170, No. 4, 15.02.2003, p. 1737-1745.

Research output: Contribution to journalArticle

Gunturi, Anasuya ; Berg, Rance E. ; Forman, James. / Preferential survival of CD8 T and NK cells expressing high levels of CD94. In: Journal of Immunology. 2003 ; Vol. 170, No. 4. pp. 1737-1745.
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