Preferred sequences for DNA recognition by the TALI helix-loop-helix proteins

Hai Ling Hsu, Lan Huang, Julia Tsou Tsan, Walter Funk, Woodring E. Wright, Jing Shan Hu, Robert E. Kingston, Richard Baer

Research output: Contribution to journalArticle

134 Citations (Scopus)

Abstract

Tumor-specific activation of the TAL1 gene is the most common genetic alteration seen in patients with T-cell acute lymphoblastic leukemia. The TAL1 gene products contain the basic helix-loop-helix (bHLH) domain, a protein dimerization and DNA-binding motif common to several known transcription factors. A binding-site selection procedure has now been used to evaluate the DNA recognition properties of TAL1. These studies demonstrate that TAL1 polypeptides do not have intrinsic DNA-binding activity, presumably because of their inability to form bHLH homodimers. However, TAL1 readily interacts with any of the known class A bHLH proteins (E12, E47, E2-2, and HEB) to form heterodimers that bind DNA in a sequence-specific manner. The TAL1 heterodimers preferentially recognize a subset of E-box elements (CANNTG) that can be represented by the consensus sequence AACAGATGGT. This consensus is composed of half-sites for recognition by the participating class A bHLH polypeptide (AACAG) and the TAL1 polypeptide (ATGGT). TAL1 heterodimers with DNA-binding activity are readily detected in nuclear extracts of Jurkat, a leukemic cell line derived from a patient with T-cell acute lymphoblastic leukemia. Hence, TAL1 is likely to bind and regulate the transcription of a unique subset of subordinate target genes, some of which may mediate the malignant function of TAL1 during T-cell leukemogenesis.

Original languageEnglish (US)
Pages (from-to)1256-1265
Number of pages10
JournalMolecular and Cellular Biology
Volume14
Issue number2
StatePublished - Feb 1994

Fingerprint

Transcription Factor 3
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
DNA
Peptides
Helix-Loop-Helix Motifs
Proteins
Protein Multimerization
E-Box Elements
Nucleotide Motifs
Consensus Sequence
Transcriptional Activation
Genes
Transcription Factors
Binding Sites
T-Lymphocytes
Cell Line
Neoplasms

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Hsu, H. L., Huang, L., Tsan, J. T., Funk, W., Wright, W. E., Hu, J. S., ... Baer, R. (1994). Preferred sequences for DNA recognition by the TALI helix-loop-helix proteins. Molecular and Cellular Biology, 14(2), 1256-1265.

Preferred sequences for DNA recognition by the TALI helix-loop-helix proteins. / Hsu, Hai Ling; Huang, Lan; Tsan, Julia Tsou; Funk, Walter; Wright, Woodring E.; Hu, Jing Shan; Kingston, Robert E.; Baer, Richard.

In: Molecular and Cellular Biology, Vol. 14, No. 2, 02.1994, p. 1256-1265.

Research output: Contribution to journalArticle

Hsu, HL, Huang, L, Tsan, JT, Funk, W, Wright, WE, Hu, JS, Kingston, RE & Baer, R 1994, 'Preferred sequences for DNA recognition by the TALI helix-loop-helix proteins', Molecular and Cellular Biology, vol. 14, no. 2, pp. 1256-1265.
Hsu HL, Huang L, Tsan JT, Funk W, Wright WE, Hu JS et al. Preferred sequences for DNA recognition by the TALI helix-loop-helix proteins. Molecular and Cellular Biology. 1994 Feb;14(2):1256-1265.
Hsu, Hai Ling ; Huang, Lan ; Tsan, Julia Tsou ; Funk, Walter ; Wright, Woodring E. ; Hu, Jing Shan ; Kingston, Robert E. ; Baer, Richard. / Preferred sequences for DNA recognition by the TALI helix-loop-helix proteins. In: Molecular and Cellular Biology. 1994 ; Vol. 14, No. 2. pp. 1256-1265.
@article{c719a86524c84426b8cb737b7286ffe0,
title = "Preferred sequences for DNA recognition by the TALI helix-loop-helix proteins",
abstract = "Tumor-specific activation of the TAL1 gene is the most common genetic alteration seen in patients with T-cell acute lymphoblastic leukemia. The TAL1 gene products contain the basic helix-loop-helix (bHLH) domain, a protein dimerization and DNA-binding motif common to several known transcription factors. A binding-site selection procedure has now been used to evaluate the DNA recognition properties of TAL1. These studies demonstrate that TAL1 polypeptides do not have intrinsic DNA-binding activity, presumably because of their inability to form bHLH homodimers. However, TAL1 readily interacts with any of the known class A bHLH proteins (E12, E47, E2-2, and HEB) to form heterodimers that bind DNA in a sequence-specific manner. The TAL1 heterodimers preferentially recognize a subset of E-box elements (CANNTG) that can be represented by the consensus sequence AACAGATGGT. This consensus is composed of half-sites for recognition by the participating class A bHLH polypeptide (AACAG) and the TAL1 polypeptide (ATGGT). TAL1 heterodimers with DNA-binding activity are readily detected in nuclear extracts of Jurkat, a leukemic cell line derived from a patient with T-cell acute lymphoblastic leukemia. Hence, TAL1 is likely to bind and regulate the transcription of a unique subset of subordinate target genes, some of which may mediate the malignant function of TAL1 during T-cell leukemogenesis.",
author = "Hsu, {Hai Ling} and Lan Huang and Tsan, {Julia Tsou} and Walter Funk and Wright, {Woodring E.} and Hu, {Jing Shan} and Kingston, {Robert E.} and Richard Baer",
year = "1994",
month = "2",
language = "English (US)",
volume = "14",
pages = "1256--1265",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "2",

}

TY - JOUR

T1 - Preferred sequences for DNA recognition by the TALI helix-loop-helix proteins

AU - Hsu, Hai Ling

AU - Huang, Lan

AU - Tsan, Julia Tsou

AU - Funk, Walter

AU - Wright, Woodring E.

AU - Hu, Jing Shan

AU - Kingston, Robert E.

AU - Baer, Richard

PY - 1994/2

Y1 - 1994/2

N2 - Tumor-specific activation of the TAL1 gene is the most common genetic alteration seen in patients with T-cell acute lymphoblastic leukemia. The TAL1 gene products contain the basic helix-loop-helix (bHLH) domain, a protein dimerization and DNA-binding motif common to several known transcription factors. A binding-site selection procedure has now been used to evaluate the DNA recognition properties of TAL1. These studies demonstrate that TAL1 polypeptides do not have intrinsic DNA-binding activity, presumably because of their inability to form bHLH homodimers. However, TAL1 readily interacts with any of the known class A bHLH proteins (E12, E47, E2-2, and HEB) to form heterodimers that bind DNA in a sequence-specific manner. The TAL1 heterodimers preferentially recognize a subset of E-box elements (CANNTG) that can be represented by the consensus sequence AACAGATGGT. This consensus is composed of half-sites for recognition by the participating class A bHLH polypeptide (AACAG) and the TAL1 polypeptide (ATGGT). TAL1 heterodimers with DNA-binding activity are readily detected in nuclear extracts of Jurkat, a leukemic cell line derived from a patient with T-cell acute lymphoblastic leukemia. Hence, TAL1 is likely to bind and regulate the transcription of a unique subset of subordinate target genes, some of which may mediate the malignant function of TAL1 during T-cell leukemogenesis.

AB - Tumor-specific activation of the TAL1 gene is the most common genetic alteration seen in patients with T-cell acute lymphoblastic leukemia. The TAL1 gene products contain the basic helix-loop-helix (bHLH) domain, a protein dimerization and DNA-binding motif common to several known transcription factors. A binding-site selection procedure has now been used to evaluate the DNA recognition properties of TAL1. These studies demonstrate that TAL1 polypeptides do not have intrinsic DNA-binding activity, presumably because of their inability to form bHLH homodimers. However, TAL1 readily interacts with any of the known class A bHLH proteins (E12, E47, E2-2, and HEB) to form heterodimers that bind DNA in a sequence-specific manner. The TAL1 heterodimers preferentially recognize a subset of E-box elements (CANNTG) that can be represented by the consensus sequence AACAGATGGT. This consensus is composed of half-sites for recognition by the participating class A bHLH polypeptide (AACAG) and the TAL1 polypeptide (ATGGT). TAL1 heterodimers with DNA-binding activity are readily detected in nuclear extracts of Jurkat, a leukemic cell line derived from a patient with T-cell acute lymphoblastic leukemia. Hence, TAL1 is likely to bind and regulate the transcription of a unique subset of subordinate target genes, some of which may mediate the malignant function of TAL1 during T-cell leukemogenesis.

UR - http://www.scopus.com/inward/record.url?scp=0028040256&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028040256&partnerID=8YFLogxK

M3 - Article

C2 - 8289805

AN - SCOPUS:0028040256

VL - 14

SP - 1256

EP - 1265

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 2

ER -