Preliminary x‐ray analysis of crystals of plasminogen activator inhibitor‐1

E. J. Goldsmith, C. Sheng-Cheng, D. E. Danley, R. D. Gerard, K. F. Geoghegan, J. Mottonen, A. Strand

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Crystals of bacterially expressed plasminogen activator inhibitor (PAI‐1) suitable for X‐ray diffraction analysis have been obtained from 8% (w/v) PEG 1500, pH 8.25. The space group is P1, and the lattice constants are a = 82.17 Å, b = 47.82 Å, c = 62.89 Å, α = 90.00°, β = 106.90°, γ = 106.84°. The diffraction limit is 2.3 Å, and the unit cell contains two molecules of PAI‐1. The crystals contain latent PAI‐1 which can be partly reactivated by exposure to denaturants.

Original languageEnglish (US)
Pages (from-to)225-227
Number of pages3
JournalProteins: Structure, Function, and Bioinformatics
Volume9
Issue number3
DOIs
StatePublished - Mar 1991

Keywords

  • N‐terminal
  • crystallography
  • latency
  • methionine
  • protein crystals
  • tissue plasminogen activator

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

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