Preparation and analysis of antigen-specific memory B cells

E. Yefenof, V. M. Sanders, E. C. Snow, R. J. Noelle, K. G. Oliver, J. W. Uhr, E. S. Vitetta

Research output: Contribution to journalArticle

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Abstract

A procedure has been developed for the enrichment of TNP-binding memory B cells (TNP-MABC) from spleens of immunized mice. More than 75% of the cells expressed surface IgM (sIgM) and IgD (sIgD) and about 9% expressed surface IgG (sIgG). The TNP-MABC consisted of small resting lymphocytes with high affinity antigen-binding receptors. These cells expressed increased densities of Ia antigens and decreased densities of sIgD. Adoptive transfer of the cells into irradiated, carrier-primed syngeneic recipients resulted in their differentiation into IgG anti-TNP antibody-secreting cells. TNP-MABC secreted high affinity IgG anti-TNP antibodies when cultured in vitro with carrier-primed T cells and antigen. Limiting dilution analysis revealed that TNP-MABC contained a relatively low frequency of precursors for IgG-secreting cells that had an exceptionally large clone size. These results show that a highly enriched population of antigen-specific memory B cells can now be prepared and used to analyze their activation requirements.

Original languageEnglish (US)
Pages (from-to)3777-3784
Number of pages8
JournalJournal of Immunology
Volume135
Issue number6
StatePublished - 1985

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B-Lymphocytes
Immunoglobulin G
Antigens
Immunoglobulin D
Anti-Idiotypic Antibodies
Activation Analysis
Antibody-Producing Cells
Antigen Receptors
Adoptive Transfer
Viral Tumor Antigens
Histocompatibility Antigens Class II
Immunoglobulin M
Spleen
Clone Cells
Lymphocytes
T-Lymphocytes
Population

ASJC Scopus subject areas

  • Immunology

Cite this

Yefenof, E., Sanders, V. M., Snow, E. C., Noelle, R. J., Oliver, K. G., Uhr, J. W., & Vitetta, E. S. (1985). Preparation and analysis of antigen-specific memory B cells. Journal of Immunology, 135(6), 3777-3784.

Preparation and analysis of antigen-specific memory B cells. / Yefenof, E.; Sanders, V. M.; Snow, E. C.; Noelle, R. J.; Oliver, K. G.; Uhr, J. W.; Vitetta, E. S.

In: Journal of Immunology, Vol. 135, No. 6, 1985, p. 3777-3784.

Research output: Contribution to journalArticle

Yefenof, E, Sanders, VM, Snow, EC, Noelle, RJ, Oliver, KG, Uhr, JW & Vitetta, ES 1985, 'Preparation and analysis of antigen-specific memory B cells', Journal of Immunology, vol. 135, no. 6, pp. 3777-3784.
Yefenof E, Sanders VM, Snow EC, Noelle RJ, Oliver KG, Uhr JW et al. Preparation and analysis of antigen-specific memory B cells. Journal of Immunology. 1985;135(6):3777-3784.
Yefenof, E. ; Sanders, V. M. ; Snow, E. C. ; Noelle, R. J. ; Oliver, K. G. ; Uhr, J. W. ; Vitetta, E. S. / Preparation and analysis of antigen-specific memory B cells. In: Journal of Immunology. 1985 ; Vol. 135, No. 6. pp. 3777-3784.
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AU - Uhr, J. W.

AU - Vitetta, E. S.

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