Preparation and characterization of novel semi-interpenetrating polymer network hydrogel microspheres of chitosan and hydroxypropyl cellulose for controlled release of chlorothiazide

A. P. Rokhade, P. V. Kulkarni, N. N. Mallikarjuna, T. M. Aminabhavi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Novel semi-interpenetrating polymer network (IPN) hydrogel microspheres of chitosan (CS) and hydroxypropyl cellulose (HPC) were prepared by emulsion-cross-linking method using glutaraldehyde (GA) as a cross-linker. Chlorothiazide (CT), a diuretic and anti-hypertensive drug with limited water solubility, was successfully encapsulated into IPN microspheres. Various formulations were prepared by varying the ratio of CS and HPC, percentage drug loading and amount of GA. Microspheres were characterized by Fourier transform infrared (FTIR) spectroscopy to investigate the formation of IPN structure and to confirm the absence of chemical interactions between drug, polymer and cross-linking agent. Scanning electron microscopy (SEM) was performed to study the surface morphology of the microspheres. SEM showed that microspheres have smooth surfaces. Particle size, as measured by laser light scattering technique, gave an average size ranging from 199-359 μm. Differential scanning calorimetry (DSC) was performed to know the formation of IPN structure. X-ray diffraction (X-RD) studies were performed to understand the crystalline nature of the drug after encapsulation into IPN microspheres. Encapsulation of drug up to 76% was achieved as measured by UV spectroscopy. Both equilibrium and dynamic swelling experiments were performed in 0.1 N HCl. Diffusion coefficients (D) for water transport through the microspheres were estimated using an empirical equation. In vitro release studies indicated the dependence of release rate on the extent of cross-linking, drug loading and the amount of HPC used to produce the microspheres; slow release was extended up to 12 h. The release data were also fitted to an empirical equation to compute the diffusional exponent (n), which indicated that the release followed the non-Fickian trend.

Original languageEnglish (US)
Pages (from-to)27-36
Number of pages10
JournalJournal of Microencapsulation
Volume26
Issue number1
DOIs
StatePublished - 2009

Fingerprint

Chlorothiazide
Interpenetrating polymer networks
Hydrogel
Chitosan
Microspheres
cellulose
Hydrogels
Cellulose
Polymers
drugs
preparation
polymers
Glutaral
diuretics
Encapsulation
Pharmaceutical Preparations
Electron Scanning Microscopy
scanning electron microscopy
Drug interactions
swelling

Keywords

  • Chlorothiazide
  • Cross-linking
  • Drug delivery systems
  • Hydrogels
  • Interpenetrating polymer networks
  • Microspheres

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Bioengineering
  • Organic Chemistry

Cite this

Preparation and characterization of novel semi-interpenetrating polymer network hydrogel microspheres of chitosan and hydroxypropyl cellulose for controlled release of chlorothiazide. / Rokhade, A. P.; Kulkarni, P. V.; Mallikarjuna, N. N.; Aminabhavi, T. M.

In: Journal of Microencapsulation, Vol. 26, No. 1, 2009, p. 27-36.

Research output: Contribution to journalArticle

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