Preparation and identification of monoclonal antibodies against snake venom C-type lectin like protein Agkisacutacin

Li Jing Su, Liang Xiao, Tao Zhan, Lan Chen, Yan Qiu Dong, L. Fang, Lian Sheng Cheng, Jing Liu

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

AIM: To prepare and identify the antibodies against snake venom C-type lectin like protein Agkisacutacin. METHODS: A BALB/c mouse was immunized with Agkisacutacin and the cells were fused by standard hybridoma technique to prepare mAbs. The stability of the hybridomas secreting mAbs was detected by indirect ELISA. The nuclear type of the hybridomas was analyzed by fluorescent staining and the specificity of mAbs was detected by Western blot. RESULTS: Three cell strains of hybridomas that could steadily secrete anti-Agkisacutacin mAb were obtained. CONCLUSION: The successful preparation of anti-Agkisacutacin mAbs provides an important tool for studying the in vivo metabolism of new anti-thrombus drugs by detecting Agkisacutacin and investigating the mechanism of anti-thrombus of the drugs.

Original languageEnglish (US)
Pages (from-to)1031-1033
Number of pages3
JournalXi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Volume23
Issue number11
StatePublished - Nov 2007
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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