Preparation of cationic nanogels for nucleic acid delivery

Saadyah E. Averick, Eduardo Paredes, Ainara Irastorza, Arun R. Shrivats, Abiraman Srinivasan, Daniel J. Siegwart, Andrew J. Magenau, Hong Y. Cho, Eric Hsu, Amram A. Averick, Jinku Kim, Shiguang Liu, Jeffrey O. Hollinger, Subha R. Das, Krzysztof Matyjaszewski

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Abstract

Cationic nanogels with site-selected functionality were designed for the delivery of nucleic acid payloads targeting numerous therapeutic applications. Functional cationic nanogels containing quaternized 2-(dimethylamino)ethyl methacrylate and a cross-linker with reducible disulfide moieties (qNG) were prepared by activators generated by electron transfer (AGET) atom transfer radical polymerization (ATRP) in an inverse miniemulsion. Polyplex formation between the qNG and nucleic acid exemplified by plasmid DNA (pDNA) and short interfering RNA (siRNA duplexes) were evaluated. The delivery of polyplexes was optimized for the delivery of pDNA and siRNA to the Drosophila Schneider 2 (S2) cell-line. The qNG/nucleic acid (i.e., siRNA and pDNA) polyplexes were found to be highly effective in their capabilities to deliver their respective payloads.

Original languageEnglish (US)
Pages (from-to)3445-3449
Number of pages5
JournalBiomacromolecules
Volume13
Issue number11
DOIs
Publication statusPublished - Nov 13 2012

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ASJC Scopus subject areas

  • Bioengineering
  • Materials Chemistry
  • Polymers and Plastics
  • Biomaterials

Cite this

Averick, S. E., Paredes, E., Irastorza, A., Shrivats, A. R., Srinivasan, A., Siegwart, D. J., ... Matyjaszewski, K. (2012). Preparation of cationic nanogels for nucleic acid delivery. Biomacromolecules, 13(11), 3445-3449. https://doi.org/10.1021/bm301166s