Preparation of cationic nanogels for nucleic acid delivery

Saadyah E. Averick; Eduardo Paredes; Ainara Irastorza; Arun R. Shrivats; Abiraman Srinivasan; Daniel J. Siegwart; Andrew J. Magenau; Hong Y. Cho; Eric Hsu; Amram A. Averick; Jinku Kim; Shiguang Liu; Jeffrey O. Hollinger; Subha R. Das; Krzysztof Matyjaszewski

doi:10.1021/bm301166s

Preparation of cationic nanogels for nucleic acid delivery

Saadyah E. Averick, Eduardo Paredes, Ainara Irastorza, Arun R. Shrivats, Abiraman Srinivasan, Daniel J. Siegwart, Andrew J. Magenau, Hong Y. Cho, Eric Hsu, Amram A. Averick, Jinku Kim, Shiguang Liu, Jeffrey O. Hollinger, Subha R. Das, Krzysztof Matyjaszewski

Research output: Contribution to journal › Article › peer-review

67 Scopus citations

Abstract

Cationic nanogels with site-selected functionality were designed for the delivery of nucleic acid payloads targeting numerous therapeutic applications. Functional cationic nanogels containing quaternized 2-(dimethylamino)ethyl methacrylate and a cross-linker with reducible disulfide moieties (qNG) were prepared by activators generated by electron transfer (AGET) atom transfer radical polymerization (ATRP) in an inverse miniemulsion. Polyplex formation between the qNG and nucleic acid exemplified by plasmid DNA (pDNA) and short interfering RNA (siRNA duplexes) were evaluated. The delivery of polyplexes was optimized for the delivery of pDNA and siRNA to the Drosophila Schneider 2 (S2) cell-line. The qNG/nucleic acid (i.e., siRNA and pDNA) polyplexes were found to be highly effective in their capabilities to deliver their respective payloads.

Original language	English (US)
Pages (from-to)	3445-3449
Number of pages	5
Journal	Biomacromolecules
Volume	13
Issue number	11
DOIs	https://doi.org/10.1021/bm301166s
State	Published - Nov 13 2012

ASJC Scopus subject areas

Bioengineering
Biomaterials
Polymers and Plastics
Materials Chemistry

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abstract = "Cationic nanogels with site-selected functionality were designed for the delivery of nucleic acid payloads targeting numerous therapeutic applications. Functional cationic nanogels containing quaternized 2-(dimethylamino)ethyl methacrylate and a cross-linker with reducible disulfide moieties (qNG) were prepared by activators generated by electron transfer (AGET) atom transfer radical polymerization (ATRP) in an inverse miniemulsion. Polyplex formation between the qNG and nucleic acid exemplified by plasmid DNA (pDNA) and short interfering RNA (siRNA duplexes) were evaluated. The delivery of polyplexes was optimized for the delivery of pDNA and siRNA to the Drosophila Schneider 2 (S2) cell-line. The qNG/nucleic acid (i.e., siRNA and pDNA) polyplexes were found to be highly effective in their capabilities to deliver their respective payloads.",

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doi = "10.1021/bm301166s",

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AU - Paredes, Eduardo

AU - Irastorza, Ainara

AU - Shrivats, Arun R.

AU - Srinivasan, Abiraman

AU - Siegwart, Daniel J.

AU - Magenau, Andrew J.

AU - Cho, Hong Y.

AU - Hsu, Eric

AU - Averick, Amram A.

AU - Kim, Jinku

AU - Liu, Shiguang

AU - Hollinger, Jeffrey O.

AU - Das, Subha R.

AU - Matyjaszewski, Krzysztof

PY - 2012/11/13

Y1 - 2012/11/13

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Preparation of cationic nanogels for nucleic acid delivery

Abstract

ASJC Scopus subject areas

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