Preparation of microsomes to study Ca2+ channels

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Native cerebellar microsomes are used to study the properties of native cerebellar inositol(1,4,5)- trisphosphate receptor 1 (InsP3R1) and ryanodine receptor 1 (RyanR1) Ca2+ release channels. Additionally, microsomes prepared from Sf9 cells infected with InsP3R- or RyanR-expressing recombinant baculoviruses can be used to compare properties of different InsP3R and RyanR isoforms and to perform structure-function studies of both types of receptors. This protocol describes how to prepare ER microsomes from native cerebellar tissue or Sf9 cells infected with InsP3R- or RyanRexpressing recombinant baculoviruses for planar lipid bilayer (also called black lipid membranes or BLM) experiments. Prepared material is aliquoted and can be stored at -80°C freezer for many months before BLM experiments.

Original languageEnglish (US)
Pages (from-to)1037-1040
Number of pages4
JournalCold Spring Harbor Protocols
Volume2013
Issue number11
DOIs
StatePublished - Nov 2013

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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