Presence of activated antigen-binding B cells during immunization enhances relative levels of IFN-γ in T cell responses

Chandrashekhar Pasare, Vivian Morafo, Maureen Entringer, Pratima Bansal, Anna George, Vineeta Bal, Satyajit Rath, Jeannine M. Durdik

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

To examine the influence of Ag presentation by B cells on immune responses, we have used mice transgenic for an Ig heavy chain from a monoclonal anti-azobenzenearsonate (Ars) Ab to deliver Ag to B cells during immunization. A large proportion of transgene-expressing B cells in these mice binds Ars, while transgenic serum Ig shows poor Ars binding. Transgenic B cells present Ars proteins better than their nonhaptenated counterparts. This is associated with an increase in the proliferative responses of transgenic T cells to Ars protein immunization. Although B cell numbers in the transgenic mice are lower, many B cells in them show an activated phenotype, as identified by altered surface levels of peanut agglutinin reactivity, CD23, CD24, CD44, CD62L, and CD86. Even against nonhaptenated immunogens, transgenic responses show significant enhancement in the relative proportions of the Th1 cytokine IFN-γ over the Th2 cytokines IL-4 and IL- 10. Haptenated immunogens further enhance the predilection of transgenic mice to produce relatively more IFN-γ. Consistent with this, there is an increase in IgG2a/IgG1 ratios in serum Abs in response to haptenated immunogens in transgenic mice. Adoptive transfer of primed hapten-specific secondary B cells into nontransgenic mice also induces an increase in relative levels of IFN-γ in response to haptenated immunogens. Thus, presentation of immunogen in vivo by activated Ag-binding B cells contributes to enhanced immunogenicity and a Th1 cytokine bias.

Original languageEnglish (US)
Pages (from-to)778-787
Number of pages10
JournalJournal of Immunology
Volume160
Issue number2
StatePublished - Jan 15 1998

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Pasare, C., Morafo, V., Entringer, M., Bansal, P., George, A., Bal, V., Rath, S., & Durdik, J. M. (1998). Presence of activated antigen-binding B cells during immunization enhances relative levels of IFN-γ in T cell responses. Journal of Immunology, 160(2), 778-787.