Presence of an Al,3 fucosyltransferase (Fuc to GlcNAc) in the helminthic parasite, Haemonchus contortus

R. Debose-Boyd, A. K. Nyâme, R. D. Cummings

Research output: Contribution to journalArticle

Abstract

We recently demonstrated that the helminthic parasite Schistosoma mansoni contains an a 1,3 fucosyltransferase (al,3FT) that causes synthesis of the Lewis x antigen (£ex) Gal#l-4(Fucal-3)GlcNAc-R and a related structure GalNAc/5l-4(FucQl-3)GlcNAc-R (GalNAc-Iex-type). This latter structure is also synthesized by the dog heartworm Diroβlaria immitis. These fucosylated glycans have been implicated in several aspects of the host-parasite interactions. To explore the possibility that other helminthic parasites contain an al,3FT. we have assayed the al,3FT activity in detergent extracts of H. Contortus, a parasitic nematode which dwells in the gut of ruminants. An extract of this parasite contains high levels of an al,3FT that efficiently uses lacto-N-neotetraose GaL3l-4GlcNAcβl-3Gal#l-4Glc as an acceptor to synthesize lacto-N-fucopentaose III Gal01-4(Fucal-3)GlcNAcβl-3Galβl-4Glc. The product was characterized by Dionex HPAEC co-chromatography with standards and by sensitivity to an al-3/al-4-specific fucosidase. Lectin binding studies also reveal that H. contortus glycoproteins contain the GalNAc-IeTtype structures. These results signify that the ul,3FT and the GalNAc-ie1type structures are general features of the biosynthetic pathways for glycoconjugates in helminths.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number6
StatePublished - 1996

Fingerprint

galactoside 3-fucosyltransferase
Haemonchus
Haemonchus contortus
Parasites
parasites
alpha-L-Fucosidase
Dirofilaria immitis
animal parasitic nematodes
Host-Parasite Interactions
heartworms
glycoconjugates
Glycoconjugates
Schistosoma mansoni
Helminths
host-parasite relationships
Biosynthetic Pathways
extracts
Ruminants
Lectins
helminths

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Presence of an Al,3 fucosyltransferase (Fuc to GlcNAc) in the helminthic parasite, Haemonchus contortus. / Debose-Boyd, R.; Nyâme, A. K.; Cummings, R. D.

In: FASEB Journal, Vol. 10, No. 6, 1996.

Research output: Contribution to journalArticle

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