Primary cell culture of human type II pneumonocytes: Maintenance of a differentiated phenotype and transfection with recombinant adenoviruses

Medicine & Life Sciences

• Primary Cell Culture 93%
• Adenoviridae 75%
• Transfection 66%
• Maintenance 54%
• Phenotype 46%
• Cultured Cells 21%
• Human Growth Hormone 21%
• Organ Culture Techniques 20%
• Cell Culture Techniques 19%
• Surface-Active Agents 17%
• Gene Expression 15%
• DNA 14%
• Air 13%
• Fetus 13%
• Galactosidases 12%
• Fibroblasts 12%
• DEAE-Dextran 12%
• Human Adenoviruses 11%
• Bucladesine 11%
• Lac Operon 10%
• Madin Darby Canine Kidney Cells 10%
• Electroporation 10%
• 5' Flanking Region 10%
• calcium phosphate 9%
• Collagenases 9%
• Islands 9%
• Lung 9%
• Cytomegalovirus 8%
• Cyclic AMP 8%
• Lipoproteins 8%
• Culture Media 7%
• Digestion 7%
• Glucocorticoids 7%
• Extracellular Matrix 7%
• Genes 6%
• Proteins 6%
• Epithelial Cells 6%
• Messenger RNA 4%

Chemical Compounds

• Growth Hormone 100%
• Electroporation 54%
• Surfactant 52%
• Glucocorticoid 49%
• Lipoprotein 42%
• Culture Media 41%
• Refractory 40%
• Protein 32%
• Pharmacological Metabolism 29%
• Calcium(0) 29%
• Amount 16%