Primary structural studies of the Qa-2 alloantigen

implications for the evolution of the MHC

Mark J. Soloski, Jonathan W. Uhr, Ellen S. Vitetta

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The murine major histocompatibility complex (MHC) contains a number of loci encoding cell-surface glycoproteins of molecular weight (MW) 40,000-45,000 which are non-covalently associated with β2-microglobulin. The products of this large multigene family, collectively referred to as class I gene products, include H-2K,-D,-L and -R, which are encoded by the H-2 complex, and Qa-1, -2 and TL, encoded by the Tla region telomeric to H-2 (refs 1-5). Functional studies have shown that the H-2K, -D and -L gene products serve as important self-recognition structures in immune responses6,7; a functional role for the other class I gene products has not been established. Tryptic peptide map comparisons and primary sequence analyses have indicated that H-2K, -D and -L are 70-85% homologous with respect to their amino acid sequences8-10. The data, however, have failed to reveal structural features unique to gene products of a given locus. Recent tryptic peptide map comparisons of the Tla gene products, Qa-2 and TL, have shown that Qa-2, H-2K and -D are more closely related to each other than to TL11,12. Here we present the NH2-terminal sequence of the Qa-2 alloantigen. Our results, together with those of previous studies, show that Qa-2 has significant homology to H-2 antigens but differs in that Qa-2 molecules lack extensive polymorphism and the Qa-2 heavy chain has two additional NH2-terminal amino acids and several critical amino acid interchanges compared with H-2 antigens.

Original languageEnglish (US)
Pages (from-to)759-761
Number of pages3
JournalNature
Volume296
Issue number5859
DOIs
StatePublished - 1982

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Isoantigens
Major Histocompatibility Complex
H-2 Antigens
MHC Class I Genes
Amino Acids
Genes
Peptides
Membrane Glycoproteins
Multigene Family
Sequence Analysis
Molecular Weight

ASJC Scopus subject areas

  • General

Cite this

Primary structural studies of the Qa-2 alloantigen : implications for the evolution of the MHC. / Soloski, Mark J.; Uhr, Jonathan W.; Vitetta, Ellen S.

In: Nature, Vol. 296, No. 5859, 1982, p. 759-761.

Research output: Contribution to journalArticle

Soloski, Mark J. ; Uhr, Jonathan W. ; Vitetta, Ellen S. / Primary structural studies of the Qa-2 alloantigen : implications for the evolution of the MHC. In: Nature. 1982 ; Vol. 296, No. 5859. pp. 759-761.
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