Primary structure, synthesis, and biological activity of rat endothelin, an endothelium-derived vasoconstrictor peptide

M. Yanagisawa, A. Inoue, T. Ishikawa, Y. Kasuya, S. Kimura, S. Kumagaye, K. Nakajima, T. X. Watanabe, S. Sakakibara, K. Goto, T. Masaki

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Abstract

Endothelin is a potent vasoconstrictor/pressor peptide, which we recently characterized from the conditioned culture medium of porcine aortic endothelial cells. We report here the cloning and partial sequencing of the rat endothelin gene. The nucleotide sequence predicted a 21-residue peptide similar to, but distinct from, porcine endothelium; 15 residues of rat endothelium were identical and 3 residues were substitutions by chemically similar amino acid residues to those in the porcine peptide. Synthetic rat endothelin was then prepared according to its deduced amino acid sequence. This synthetic peptide had (i) potent vasoconstrictor activity in the rat aortic strip and in perfused rat heart and (ii) a characteristically long-lasting in vivo pressor activity by intraaortic bolus injection in the conscious rat.

Original languageEnglish (US)
Pages (from-to)6964-6967
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number18
StatePublished - 1988

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Endothelins
Vasoconstrictor Agents
Endothelium
Peptides
Swine
Conditioned Culture Medium
Organism Cloning
Amino Acid Sequence
Endothelial Cells
Amino Acids
Injections
Genes

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Primary structure, synthesis, and biological activity of rat endothelin, an endothelium-derived vasoconstrictor peptide. / Yanagisawa, M.; Inoue, A.; Ishikawa, T.; Kasuya, Y.; Kimura, S.; Kumagaye, S.; Nakajima, K.; Watanabe, T. X.; Sakakibara, S.; Goto, K.; Masaki, T.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, No. 18, 1988, p. 6964-6967.

Research output: Contribution to journalArticle

Yanagisawa, M, Inoue, A, Ishikawa, T, Kasuya, Y, Kimura, S, Kumagaye, S, Nakajima, K, Watanabe, TX, Sakakibara, S, Goto, K & Masaki, T 1988, 'Primary structure, synthesis, and biological activity of rat endothelin, an endothelium-derived vasoconstrictor peptide', Proceedings of the National Academy of Sciences of the United States of America, vol. 85, no. 18, pp. 6964-6967.
Yanagisawa, M. ; Inoue, A. ; Ishikawa, T. ; Kasuya, Y. ; Kimura, S. ; Kumagaye, S. ; Nakajima, K. ; Watanabe, T. X. ; Sakakibara, S. ; Goto, K. ; Masaki, T. / Primary structure, synthesis, and biological activity of rat endothelin, an endothelium-derived vasoconstrictor peptide. In: Proceedings of the National Academy of Sciences of the United States of America. 1988 ; Vol. 85, No. 18. pp. 6964-6967.
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AU - Inoue, A.

AU - Ishikawa, T.

AU - Kasuya, Y.

AU - Kimura, S.

AU - Kumagaye, S.

AU - Nakajima, K.

AU - Watanabe, T. X.

AU - Sakakibara, S.

AU - Goto, K.

AU - Masaki, T.

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AB - Endothelin is a potent vasoconstrictor/pressor peptide, which we recently characterized from the conditioned culture medium of porcine aortic endothelial cells. We report here the cloning and partial sequencing of the rat endothelin gene. The nucleotide sequence predicted a 21-residue peptide similar to, but distinct from, porcine endothelium; 15 residues of rat endothelium were identical and 3 residues were substitutions by chemically similar amino acid residues to those in the porcine peptide. Synthetic rat endothelin was then prepared according to its deduced amino acid sequence. This synthetic peptide had (i) potent vasoconstrictor activity in the rat aortic strip and in perfused rat heart and (ii) a characteristically long-lasting in vivo pressor activity by intraaortic bolus injection in the conscious rat.

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