Probing carbohydrate metabolism using hyperpolarized 13C-labeled molecules

Jaspal Singh, Eul Hyun Suh, Gaurav Sharma, Chalermchai Khemtong, Dean Sherry, Zoltan Kovacs

Research output: Contribution to journalArticle

Abstract

Glycolysis is a fundamental metabolic process in all organisms. Anomalies in glucose metabolism are linked to various pathological conditions. In particular, elevated aerobic glycolysis is a characteristic feature of rapidly growing cells. Glycolysis and the closely related pentose phosphate pathway can be monitored in real time by hyperpolarized 13C-labeled metabolic substrates such as 13C-enriched, deuterated D-glucose derivatives, [2-13C]-D-fructose, [2-13C] dihydroxyacetone, [1-13C]-D-glycerate, [1-13C]-D-glucono-δ-lactone and [1-13C] pyruvate in healthy and diseased tissues. Elevated glycolysis in tumors (the Warburg effect) was also successfully imaged using hyperpolarized [U-13C6, U-2H7]-D-glucose, while the size of the preexisting lactate pool can be measured by 13C MRS and/or MRI with hyperpolarized [1-13C]pyruvate. This review summarizes the application of various hyperpolarized 13C-labeled metabolites to the real-time monitoring of glycolysis and related metabolic processes in normal and diseased tissues.

Original languageEnglish (US)
Article numbere4018
JournalNMR in Biomedicine
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Carbohydrate Metabolism
Glycolysis
Molecules
Pyruvic Acid
Glucose
Dihydroxyacetone
Tissue
Pentoses
Pentose Phosphate Pathway
Lactones
Metabolites
Fructose
Metabolism
Magnetic resonance imaging
Tumors
Lactic Acid
Phosphates
Derivatives
Monitoring
Substrates

Keywords

  • dynamic nuclear polarization
  • glycolysis
  • hyperpolarized C NMR
  • metabolic probes

ASJC Scopus subject areas

  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Spectroscopy

Cite this

Probing carbohydrate metabolism using hyperpolarized 13C-labeled molecules. / Singh, Jaspal; Suh, Eul Hyun; Sharma, Gaurav; Khemtong, Chalermchai; Sherry, Dean; Kovacs, Zoltan.

In: NMR in Biomedicine, 01.01.2018.

Research output: Contribution to journalArticle

@article{5271764beadd49eab494c903fdd16e70,
title = "Probing carbohydrate metabolism using hyperpolarized 13C-labeled molecules",
abstract = "Glycolysis is a fundamental metabolic process in all organisms. Anomalies in glucose metabolism are linked to various pathological conditions. In particular, elevated aerobic glycolysis is a characteristic feature of rapidly growing cells. Glycolysis and the closely related pentose phosphate pathway can be monitored in real time by hyperpolarized 13C-labeled metabolic substrates such as 13C-enriched, deuterated D-glucose derivatives, [2-13C]-D-fructose, [2-13C] dihydroxyacetone, [1-13C]-D-glycerate, [1-13C]-D-glucono-δ-lactone and [1-13C] pyruvate in healthy and diseased tissues. Elevated glycolysis in tumors (the Warburg effect) was also successfully imaged using hyperpolarized [U-13C6, U-2H7]-D-glucose, while the size of the preexisting lactate pool can be measured by 13C MRS and/or MRI with hyperpolarized [1-13C]pyruvate. This review summarizes the application of various hyperpolarized 13C-labeled metabolites to the real-time monitoring of glycolysis and related metabolic processes in normal and diseased tissues.",
keywords = "dynamic nuclear polarization, glycolysis, hyperpolarized C NMR, metabolic probes",
author = "Jaspal Singh and Suh, {Eul Hyun} and Gaurav Sharma and Chalermchai Khemtong and Dean Sherry and Zoltan Kovacs",
year = "2018",
month = "1",
day = "1",
doi = "10.1002/nbm.4018",
language = "English (US)",
journal = "NMR in Biomedicine",
issn = "0952-3480",
publisher = "John Wiley and Sons Ltd",

}

TY - JOUR

T1 - Probing carbohydrate metabolism using hyperpolarized 13C-labeled molecules

AU - Singh, Jaspal

AU - Suh, Eul Hyun

AU - Sharma, Gaurav

AU - Khemtong, Chalermchai

AU - Sherry, Dean

AU - Kovacs, Zoltan

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Glycolysis is a fundamental metabolic process in all organisms. Anomalies in glucose metabolism are linked to various pathological conditions. In particular, elevated aerobic glycolysis is a characteristic feature of rapidly growing cells. Glycolysis and the closely related pentose phosphate pathway can be monitored in real time by hyperpolarized 13C-labeled metabolic substrates such as 13C-enriched, deuterated D-glucose derivatives, [2-13C]-D-fructose, [2-13C] dihydroxyacetone, [1-13C]-D-glycerate, [1-13C]-D-glucono-δ-lactone and [1-13C] pyruvate in healthy and diseased tissues. Elevated glycolysis in tumors (the Warburg effect) was also successfully imaged using hyperpolarized [U-13C6, U-2H7]-D-glucose, while the size of the preexisting lactate pool can be measured by 13C MRS and/or MRI with hyperpolarized [1-13C]pyruvate. This review summarizes the application of various hyperpolarized 13C-labeled metabolites to the real-time monitoring of glycolysis and related metabolic processes in normal and diseased tissues.

AB - Glycolysis is a fundamental metabolic process in all organisms. Anomalies in glucose metabolism are linked to various pathological conditions. In particular, elevated aerobic glycolysis is a characteristic feature of rapidly growing cells. Glycolysis and the closely related pentose phosphate pathway can be monitored in real time by hyperpolarized 13C-labeled metabolic substrates such as 13C-enriched, deuterated D-glucose derivatives, [2-13C]-D-fructose, [2-13C] dihydroxyacetone, [1-13C]-D-glycerate, [1-13C]-D-glucono-δ-lactone and [1-13C] pyruvate in healthy and diseased tissues. Elevated glycolysis in tumors (the Warburg effect) was also successfully imaged using hyperpolarized [U-13C6, U-2H7]-D-glucose, while the size of the preexisting lactate pool can be measured by 13C MRS and/or MRI with hyperpolarized [1-13C]pyruvate. This review summarizes the application of various hyperpolarized 13C-labeled metabolites to the real-time monitoring of glycolysis and related metabolic processes in normal and diseased tissues.

KW - dynamic nuclear polarization

KW - glycolysis

KW - hyperpolarized C NMR

KW - metabolic probes

UR - http://www.scopus.com/inward/record.url?scp=85057270099&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85057270099&partnerID=8YFLogxK

U2 - 10.1002/nbm.4018

DO - 10.1002/nbm.4018

M3 - Article

C2 - 30474153

AN - SCOPUS:85057270099

JO - NMR in Biomedicine

JF - NMR in Biomedicine

SN - 0952-3480

M1 - e4018

ER -