Probing lipase/esterase libraries for lipid A hydrolases - Discovery of biocatalysts for the detoxification of bacterially-expressed recombinant protein

Jung Mo Ahn, Paul Wentworth, Kim D. Janda

Research output: Contribution to journalArticle

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Abstract

In our ongoing efforts to develop new methods for lipopolysaccharide (LPS) detoxification, we have screened lipase/ esterase libraries for the ability to deacylate the 2′- and 3′-fatty acid chains from lipid A: the most active esterases were successfully employed to inactivate LPSs in a crude concentrated cell supernatant of E. Coli containing a recombinant single chain antibody (scFv).

Original languageEnglish (US)
Pages (from-to)364-365
Number of pages2
JournalChemical Communications
Volume10
Issue number4
Publication statusPublished - Feb 21 2004

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ASJC Scopus subject areas

  • Chemistry(all)

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