Probing lipase/esterase libraries for lipid A hydrolases—discovery of biocatalysts for the detoxification of bacterially-expressed recombinant protein

Jung Mo Ahn, Paul Wentworth, Kim D. Janda

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

In our ongoing efforts to develop new methods for lipopolysaccharide (LPS) detoxification, we have screened lipase/esterase libraries for the ability to deacylate the 2’- and 3’-fatty acid chains from lipid A: the most active esterases were successfully employed to inactivate LPSs in a crude concentrated cell supernatant of E. Coli containing a recombinant single chain antibody (scFv).

Original languageEnglish (US)
Pages (from-to)364-365
Number of pages2
JournalChemical Communications
Volume4
Issue number4
DOIs
StatePublished - Jan 1 2004

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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