Probing the structure of cytoplasm

K. Luby-Phelps, D. L. Taylor, F. Lanni

Research output: Contribution to journalArticle

229 Scopus citations

Abstract

We have used size-fractionated, fluorescent dextrans to probe the structure of the cytoplasmic ground substance of living Swiss 3T3 cells by fluorescence recovery after photobleaching and video image processing. The data indicate that the cytoplasm of living cells has a fluid phase viscosity four times greater than water and contains structural barriers that restrict free diffusion of dissolved macromolecules in a size-dependent manner. Assuming these structural barriers comprise a filamentous meshwork, the combined fluorescence recovery after photobleaching and imaging data suggest that the average pore size of the meshwork is in the range of 300 to 400 Å, but may be as small as 200 Å in some cytoplasmic domains.

Original languageEnglish (US)
Pages (from-to)2015-2022
Number of pages8
JournalJournal of Cell Biology
Volume102
Issue number6
DOIs
StatePublished - 1986

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'Probing the structure of cytoplasm'. Together they form a unique fingerprint.

  • Cite this